Species-specific chemosensory gene expression in the olfactory organs of the malaria vector Anopheles gambiae.
Species-specific chemosensory gene expression in the olfactory organs of the malaria vector Anopheles gambiae.
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DOI：
10.1186/1471-2164-15-1089
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发表时间：
2014-12-11
期刊：
影响因子：
4.4
通讯作者：
Slotman MA
中科院分区：
文献类型：
--
作者：
Hodges TK;Cosme LV;Athrey G;Pathikonda S;Takken W;Slotman MA
疟蚊冈比亚按蚊（Anopheles gambiae）对人类宿主有高度偏好，这一特征极大地促进了它传播人类疟疾的能力。一个兄弟物种，安。四轮虫对寄主有不同的偏好，主要以牛科动物为食。因此，它不会促进人类疟疾传播。蚊子寻找寄主是由嗅觉系统调节的，嗅觉系统主要位于触须和上颌触须。因此，兄弟物种对不同宿主气味的检测可能反映在相关嗅觉基因的表达水平上。因此，我们比较了安的触角和上颌触须的转录组。冈比亚和安哥拉。quadriannulatus。我们发现了7个相对丰富的嗅觉受体，9个离子型受体和3个气味结合蛋白在An中大幅上调。冈比亚按蚊的天线。有趣的是，我们发现安。冈比亚虫含有一种物种特有的嗅觉受体Or52和5个An。冈比亚特有的味觉受体（AgGr48-52）相对丰富。这五种味觉受体也在An中表达。冈比亚的触角，虽然水平较低，表明可能在嗅觉中发挥作用，而不是味觉。我们还记录了大约三倍高的嗅觉基因在上颌触须的总体表达。四环鼻，表明该器官在该物种的嗅觉系统中起重要作用。最后，二氧化碳受体基因的表达在嗜兽的安中要高出5到6倍。这意味着探测二氧化碳的灵敏度要高得多。这些结果确定了疟疾病媒An中潜在的人类宿主偏好基因。冈比亚按蚊。有趣的是，嗅觉器官中几种味觉受体的物种特异性表达表明它们在嗅觉而不是味觉中起作用。此外，上颌触须在嗅觉方面的作用比以前认为的更广泛，尽管在嗜兽猿人中更为如此。quadriannulatus。本文的在线版本（doi:10.1186/1471-2164-15-1089）包含补充材料，可供授权用户使用。
The malaria mosquito Anopheles gambiae has a high preference for human hosts, a characteristic that contributes greatly to its capacity for transmitting human malaria. A sibling species, An. quadriannulatus, has a quite different host preference and feeds mostly on bovids. For this reason it does not contribute to human malaria transmission. Host seeking in mosquitoes is modulated by the olfactory system, which is primarily housed in the antennae and maxillary palps. Therefore, the detection of differing host odors by sibling species may be reflected in the expression level of the olfactory genes involved. Accordingly, we compared the transcriptomes of the antennae and maxillary palps of An. gambiae and An. quadriannulatus. We identified seven relatively abundant olfactory receptors, nine ionotropic receptors and three odorant binding proteins that are substantially up-regulated in An. gambiae antennae. Interestingly, we find that the maxillary palps of An. gambiae contain a species-specific olfactory receptor, Or52, and five An. gambiae-specific gustatory receptors (AgGr48-52) that are relatively abundant. These five gustatory receptors are also expressed in An. gambiae antennae, although at lower level, indicating a likely role in olfaction, rather than gustation. We also document an approximately three-fold higher overall expression of olfaction genes in the maxillary palps of An. quadriannulatus, indicating an important role of this organ in the olfaction system of this species. Finally, the expression of the CO2 receptor genes is five to six-fold higher in the zoophilic An. quadriannulatus, implying a much higher sensitivity for detecting CO2. These results identify potential human host preference genes in the malaria vector An. gambiae. Interestingly, species-specific expression of several gustatory receptors in the olfactory organs indicate a role in olfaction rather than gustation. Additionally, a more expansive role for maxillary palps in olfaction is implicated than previously thought, albeit more so in the zoophilic An. quadriannulatus. The online version of this article (doi:10.1186/1471-2164-15-1089) contains supplementary material, which is available to authorized users.
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影响因子：
2.2
作者：
Li, ZX;Pickett, JA;Zhou, JJ
通讯作者：
Zhou, JJ
DOI：
10.1073/pnas.0608424104
发表时间：
2007-03-20
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
9.2
作者：
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通讯作者：
Hansson, BS
影响因子：
9.2
作者：
Lu, Tan;Qiu, Yu Tong;Zwiebell, Laurence J.
通讯作者：
Zwiebell, Laurence J.