Transcriptional reprogramming underpins enhanced plant growth promotion by the biocontrol fungus Trichoderma hamatum GD12 during antagonistic interactions with Sclerotinia sclerotiorum in soil.
Transcriptional reprogramming underpins enhanced plant growth promotion by the biocontrol fungus Trichoderma hamatum GD12 during antagonistic interactions with Sclerotinia sclerotiorum in soil.
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DOI：
10.1111/mpp.12429
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发表时间：
2016-12
影响因子：
4.9
通讯作者：
Grant MR
中科院分区：
文献类型：
--
作者：
Shaw S;Le Cocq K;Paszkiewicz K;Moore K;Winsbury R;de Torres Zabala M;Studholme DJ;Salmon D;Thornton CR;Grant MR
自由生活的土壤真菌钩状木霉菌株GD 12在木霉菌株中在控制植物病害和刺激植物生长方面都是值得注意的，这一特性在其与土壤中病原体的拮抗相互作用期间得到增强。与其他生物防治和植物生长促进木霉菌株相比，这些属性以及其显著扩展的基因组和蛋白质组，意味着合成农药和肥料的可持续替代品在控制植物病害和提高产量方面具有丰富的潜力。本研究的目的是研究GD 12在与土壤中的病原菌核盘菌（Sclerotinia sclerotiorum）的拮抗作用中的转录响应，以支持其生防和植物生长促进能力。使用广泛的mRNA-seq研究捕获土壤中病原体-拮抗剂相互作用期间的不同时间点，我们表明GD 12转录组中的动态和双相特征支持其生物防治和植物（莴苣）生长促进活性。差异表达基因的功能预测表明，在这两个过程中参与运输和氧化还原反应的蛋白质编码转录本的富集和铁载体的过度表达。我们确定了生物防治期间的双相反应，其特征在于显著诱导编码小分泌的富含半胱氨酸蛋白质的转录物、次级代谢产物产生基因簇和GD 12特有的基因。这些数据支持的假设，即核盘菌的生物防治是由合成和分泌的抗真菌化合物介导的，GD 12的独特的水库的非特征基因积极招募期间的多食性植物病原体的有效生物控制。
The free‐living soil fungus Trichoderma hamatum strain GD12 is notable amongst Trichoderma strains in both controlling plant diseases and stimulating plant growth, a property enhanced during its antagonistic interactions with pathogens in soil. These attributes, alongside its markedly expanded genome and proteome compared with other biocontrol and plant growth‐promoting Trichoderma strains, imply a rich potential for sustainable alternatives to synthetic pesticides and fertilizers for the control of plant disease and for increasing yields. The purpose of this study was to investigate the transcriptional responses of GD12 underpinning its biocontrol and plant growth promotion capabilities during antagonistic interactions with the pathogen Sclerotinia sclerotiorum in soil. Using an extensive mRNA‐seq study capturing different time points during the pathogen–antagonist interaction in soil, we show that dynamic and biphasic signatures in the GD12 transcriptome underpin its biocontrol and plant (lettuce) growth‐promoting activities. Functional predictions of differentially expressed genes demonstrate the enrichment of transcripts encoding proteins involved in transportation and oxidation–reduction reactions during both processes and an over‐representation of siderophores. We identify a biphasic response during biocontrol characterized by a significant induction of transcripts encoding small‐secreted cysteine‐rich proteins, secondary metabolite‐producing gene clusters and genes unique to GD12. These data support the hypothesis that Sclerotinia biocontrol is mediated by the synthesis and secretion of antifungal compounds and that GD12's unique reservoir of uncharacterized genes is actively recruited during the effective biological control of a plurivorous plant pathogen.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
14.9
作者：
Blin K;Medema MH;Kazempour D;Fischbach MA;Breitling R;Takano E;Weber T
通讯作者：
Weber T
DOI：
10.3763/ijas.2009.0419
发表时间：
2009-05-01
影响因子：
3.4
作者：
Hillier, Jonathan;Hawes, Cathy;Smith, Pete
通讯作者：
Smith, Pete
影响因子：
2.8
作者：
Baker, Scott E.;Perrone, Giancarlo;Kubicek, Christian P.
通讯作者：
Kubicek, Christian P.