The early development and physiology of Xenopus laevis tadpole lateral line system.
The early development and physiology of Xenopus laevis tadpole lateral line system.
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DOI：
10.1152/jn.00618.2020
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发表时间：
2021-11-01
影响因子：
2.5
通讯作者：
Li WC
中科院分区：
文献类型：
--
作者：
Saccomanno V;Love H;Sylvester A;Li WC
非洲爪蟾在其整个生命周期中具有侧线机械感觉系统，并且先前对预喂食阶段蝌蚪的研究表明，它可能在对水吸力和水射流的运动反应中发挥作用。在这里，我们调查的生理前侧线系统在新孵化的蝌蚪和其激活引起的运动输出响应短暂的吸力刺激。高速摄像显示，当头部附近施加强吸力时，蝌蚪倾向于转身游走。用DASPEI染色显示侧线神经瘤，新霉素灭活后消除了蝌蚪对吸力的运动反应。在固定的准备，抽吸或电刺激前侧线神经可靠地启动游泳，但运动神经放电涉及转向只是偶尔观察。在进行中的虚构游泳期间施加的相同刺激产生了停止反应。前侧线神经在休息时表现出自发传入放电，在刺激时活动增加。传出活动只记录在蝌蚪虚构的游泳，并在很大程度上与同侧运动神经放电同步。最后，钙成像确定了在后脑和中脑中具有荧光增加的神经元与抽吸刺激时间锁定。一群神经元在前侧线神经在背外侧后脑的入口点有最短的潜伏期在他们的反应，支持他们潜在的感觉中间神经元的身份。未来的研究需要揭示侧线感觉信息是如何被中央回路处理以决定蝌蚪运动行为的。新&值得注意的是，我们研究了非洲爪蟾蝌蚪运动反应前侧线刺激使用高速视频，电生理学和钙成像。激活侧线，可靠的开始了游泳。在高刺激强度，转向观察行为，但合适的运动神经放电被视为只是偶尔在固定蝌蚪。在游泳过程中施加的吸力产生了停止反应。我们分析了蝌蚪前外侧线神经的传入和传出活动，并使用钙成像定位感觉中间神经元。
Xenopus laevis has a lateral line mechanosensory system throughout its full life cycle, and a previous study on prefeeding stage tadpoles revealed that it may play a role in motor responses to both water suction and water jets. Here, we investigated the physiology of the anterior lateral line system in newly hatched tadpoles and the motor outputs induced by its activation in response to brief suction stimuli. High-speed videoing showed tadpoles tended to turn and swim away when strong suction was applied close to the head. The lateral line neuromasts were revealed by using DASPEI staining, and their inactivation with neomycin eliminated tadpole motor responses to suction. In immobilized preparations, suction or electrically stimulating the anterior lateral line nerve reliably initiated swimming but the motor nerve discharges implicating turning was observed only occasionally. The same stimulation applied during ongoing fictive swimming produced a halting response. The anterior lateral line nerve showed spontaneous afferent discharges at rest and increased activity during stimulation. Efferent activities were only recorded during tadpole fictive swimming and were largely synchronous with the ipsilateral motor nerve discharges. Finally, calcium imaging identified neurons with fluorescence increase time-locked with suction stimulation in the hindbrain and midbrain. A cluster of neurons at the entry point of the anterior lateral line nerve in the dorsolateral hindbrain had the shortest latency in their responses, supporting their potential sensory interneuron identity. Future studies need to reveal how the lateral line sensory information is processed by the central circuit to determine tadpole motor behavior. NEW & NOTEWORTHY We studied Xenopus tadpole motor responses to anterior lateral line stimulation using high-speed videos, electrophysiology and calcium imaging. Activating the lateral line reliably started swimming. At high stimulation intensities, turning was observed behaviorally but suitable motor nerve discharges were seen only occasionally in immobilized tadpoles. Suction applied during swimming produced a halting response. We analyzed afferent and efferent activities of the tadpole anterior lateral line nerve and located sensory interneurons using calcium imaging.
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影响因子：
5.5
作者：
CLARKE, JDW;HAYES, BP;ROBERTS, A
通讯作者：
ROBERTS, A
DOI：
10.1007/2506_2013_15
发表时间：
2014-01-01
期刊：
LATERAL LINE SYSTEM
影响因子：
--
作者：
Chagnaud, Boris Philippe;Coombs, Sheryl
通讯作者：
Coombs, Sheryl
影响因子：
2.8
作者：
Carreno, Carrie A.;Nishikawa, Kiisa C.
通讯作者：
Nishikawa, Kiisa C.
DOI：
10.1007/978-3-319-21059-9_18
发表时间：
2016-01-01
期刊：
FISH HEARING AND BIOACOUSTICS: AN ANTHOLOGY IN HONOR OF ARTHUR N. POPPER AND RICHARD R. FAY
影响因子：
--
作者：
Coffin, Allison B.;Ramcharitar, John
通讯作者：
Ramcharitar, John
影响因子：
5.3
作者：
CHASE, MH;WILLS, N
通讯作者：
WILLS, N