Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide.
Expressional induction of Paralichthys olivaceus cathepsin B gene in response to virus, poly I:C and lipopolysaccharide.
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牙鲆组织蛋白酶 B 基因响应病毒、多聚 I:C 和脂多糖的表达诱导
DOI：
10.1016/j.fsi.2008.07.018
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发表时间：
2008-11
影响因子：
4.7
通讯作者：
Gui JF
中科院分区：
文献类型：
--
作者：
Zhang FT;Zhang YB;Chen YD;Zhu R;Dong CW;Li YY;Zhang QY;Gui JF
Cathepsin B is a lysosomal cysteine protease of the papain-like enzyme family with multiple biological functions. In this study, Paralichthys olivaceus cathepsin B (PoCatB) cDNA was isolated from flounder embryonic cells (FEC) treated with UV-inactivated grass carp hemorrhage virus (GCHV) and subsequently identified as a virally induced gene. The full length cDNA of PoCatB is 1801 bp encoding 330-amino acids. The deduced protein has high homology to all known cathepsin B proteins, containing an N-terminal signal peptide, cysteine protease active sites, the occluding loop segment and a glycosylation site, all of which are conserved in the cathepsin B family. PoCatB transcription of FEC cells could be induced by turbot (Scophthalmus maximus) rhabdovirus (SMRV), UV-inactivated SMRV, UV-inactivated GCHV, poly I:C or lipopolysaccharide (LPS), and SMRV or poly I:C was revealed to be most effective among the five inducers. In normal flounder, PoCatB mRNA was detectable in all examined tissues. Moreover, SMRV infection could result in significant upregulation of PoCatB mRNA, predominantly in spleen, head kidney, posterior kidney, intestine, gill and muscle with 18.2, 10.9, 24.7, 12, 31.5 and 18 fold increases at 72 h post-infection respectively. These results provided the first evidence for the transcriptional induction of cathepsin B in fish by virus and LPS, indicating existence of a novel function in viral defense.
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DOI：
10.1073/pnas.90.7.3063
发表时间：
1993-04-01
影响因子：
11.1
作者：
KARRER, KM;PEIFFER, SL;DITOMAS, ME
通讯作者：
DITOMAS, ME
影响因子：
1.4
作者：
Chen, SL;Ren, GC;Shi, CY
通讯作者：
Shi, CY
DOI：
10.1016/j.bbrc.2005.07.143
发表时间：
2005-09-30
影响因子：
3.1
作者：
Chen, YD;Zhang, YB;Gui, JF
通讯作者：
Gui, JF
影响因子：
2.9
作者：
Aranishi, F;Mano, N;Hirose, H
通讯作者：
Hirose, H
影响因子：
1.9
作者：
Aranishi, F;Mano, N
通讯作者：
Mano, N