BZR1 Physically Interacts with SPL9 to Regulate the Vegetative Phase Change and Cell Elongation in Arabidopsis.
BZR1 Physically Interacts with SPL9 to Regulate the Vegetative Phase Change and Cell Elongation in Arabidopsis.
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DOI：
10.3390/ijms221910415
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发表时间：
2021-09-27
影响因子：
5.6
通讯作者：
Fan M
中科院分区：
文献类型：
--
作者：
Wang L;Yu P;Lyu J;Hu Y;Han C;Bai MY;Fan M
作为固着生物，植物的发育阶段转换对于植物的适应、生存和繁殖至关重要。从幼年到成年阶段的过渡-被称为营养阶段的变化-显着的内源性和环境信号的数量的影响。在这里，我们表明，油菜素类固醇（BR），一个主要的生长促进类固醇激素，积极调节拟南芥的营养阶段的变化。BR缺失突变体det 2 -1和BR不敏感突变体bri 1 -301表现出叶片宽长比增加和叶片基角减小。植物特异性转录因子类鳞状细胞启动子结合蛋白（SPL）是植物营养生长期转换的关键主控因子。BR处理显著诱导了SPL 9、SPL 10和SPL 15的表达水平，但与野生型植物相比，bri 1 -116突变体的表达水平降低。功能获得型pSPL 9：rSPL 9转基因植株表现出对下胚轴伸长的BR敏感性，并部分抑制了det 2 -1和bri 1 -301的延迟营养期转变。此外，我们发现BR信号通路的主转录因子BRASSINAZOLE-RESISTANT 1（BZR 1）与SPL 9相互作用，协同调节下游基因的表达。我们的研究结果揭示了BR通过在转录和转录后水平调节SPL 9的活性在促进营养相转变中的重要作用。
As sessile organisms, the precise development phase transitions are very important for the success of plant adaptability, survival and reproduction. The transition from juvenile to the adult phase—referred to as the vegetative phase change—is significantly influenced by numbers of endogenous and environmental signals. Here, we showed that brassinosteroid (BR), a major growth-promoting steroid hormone, positively regulates the vegetative phase change in Arabidopsis thaliana. The BR-deficient mutant det2-1 and BR-insensitive mutant bri1-301 displayed the increased ratio of leaf width to length and reduced blade base angle. The plant specific transcription factors SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) are key masters for the vegetative phase transition in plants. The expression levels of SPL9, SPL10 and SPL15 were significantly induced by BR treatment, but reduced in bri1-116 mutant compared to wild-type plants. The gain-of-function pSPL9:rSPL9 transgenic plants displayed the BR hypersensitivity on hypocotyl elongation and partially suppressed the delayed vegetative phase change of det2-1 and bri1-301. Furthermore, we showed that BRASSINAZOLE-RESISTANT 1 (BZR1), the master transcription factor of BR signaling pathway, interacted with SPL9 to cooperatively regulate the expression of downstream genes. Our findings reveal an important role for BRs in promoting vegetative phase transition through regulating the activity of SPL9 at transcriptional and post-transcriptional levels.
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影响因子：
4.5
作者：
He J;Xu M;Willmann MR;McCormick K;Hu T;Yang L;Starker CG;Voytas DF;Meyers BC;Poethig RS
通讯作者：
Poethig RS
影响因子：
21.3
作者：
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影响因子：
64.5
作者：
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通讯作者：
Walker, JC
影响因子：
16
作者：
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通讯作者：
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影响因子：
56.9
作者：
Li, JM;Nagpal, P;Chory, J
通讯作者：
Chory, J