The evolutionary conservation of the A Disintegrin-like and Metalloproteinase domain with Thrombospondin-1 motif metzincins across vertebrate species and their expression in teleost zebrafish.
The evolutionary conservation of the A Disintegrin-like and Metalloproteinase domain with Thrombospondin-1 motif metzincins across vertebrate species and their expression in teleost zebrafish.
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DOI：
10.1186/s12862-015-0281-9
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发表时间：
2015-02-15
影响因子：
3.4
通讯作者：
McCulloch DR
中科院分区：
文献类型：
--
作者：
Brunet FG;Fraser FW;Binder MJ;Smith AD;Kintakas C;Dancevic CM;Ward AC;McCulloch DR
具有血小板反应蛋白-1基序的A去整合素样和金属蛋白酶结构域（ADAMTS）酶包括19种哺乳动物锌依赖性金属蛋白酶（metzincins），其在广泛的无脊椎动物中具有同源物。ADAMTS酶由于其细胞外基质重塑活性而在发育和疾病中具有广泛的功能。在这里，我们报告了它们在脊椎动物中进化保护的详细特征。使用补充有从头测序的生物信息学，从多种生物体获得ADAMTS酶的基因序列。详细的进化分析显示，在脊椎动物中的高水平的保护与ADAMTS基因扩增的证据在两轮全基因组复制（WGD）在脊椎动物中，而串联重复事件和基因丢失也是显而易见的。然而，额外一轮的硬骨鱼特异性WGD对ADAMTS基因家族成员没有显著影响，表明它们在硬骨鱼中的保守作用保持不变。定量逆转录聚合酶链反应分析显示，动态表达的adamts基因在整个斑马鱼胚胎发育反映了关键的保守作用，他们在脊椎动物胚胎发生。值得注意的是，几个adamts mRNA的母系表达与合子表达和器官发生相一致的mRNA水平的急剧增加。广泛的adamts mRNA表达也证明在几个成年器官中，表明在成人体内平衡的潜在作用。我们的数据突出了ADAMTS基因家族的进化，通过跨后生动物的复制过程，辅之以通过脊椎动物WGD事件的扩增爆发。这也有力地假定斑马鱼作为一个潜在的模式物种，以进一步阐明ADAMTS酶在脊椎动物发育过程中的功能。本文的在线版本（doi：10.1186/s12862-015-0281-9）包含补充材料，可供授权用户使用。
The A Disintegrin-like and Metalloproteinase domain with Thrombospondin-1 motifs (ADAMTS) enzymes comprise 19 mammalian zinc-dependent metalloproteinases (metzincins) with homologues in a wide range of invertebrates. ADAMTS enzymes have a broad range of functions in development and diseases due to their extracellular matrix remodelling activity. Here, we report a detailed characterisation of their evolutionary conservation across vertebrates. Using bioinformatics complemented with de novo sequencing, gene sequences for ADAMTS enzymes were obtained from a variety of organisms. Detailed evolutionary analyses revealed a high level of conservation across vertebrates with evidence of ADAMTS gene expansion during two rounds of whole genome duplication (WGD) in vertebrates, while tandem duplication events and gene loss were also apparent. However, the additional round of teleost-specific WGD did not have a significant effect on ADAMTS gene family members suggesting their conserved roles have remained constant in teleost fish. Quantitative reverse-transcriptase polymerase chain reaction analysis revealed dynamic expression of adamts genes throughout zebrafish embryonic development reflecting the key conserved roles they play in vertebrate embryogenesis. Notably, several adamts mRNAs were maternally expressed with a dramatic increase in mRNA levels coinciding with zygotic expression and organogenesis. Broad adamts mRNA expression was also demonstrated in several adult organs indicating potential roles in adult homeostasis. Our data highlight the evolution of the ADAMTS gene family through duplication processes across metazoans supplemented by a burst of amplification through vertebrate WGD events. It also strongly posits the zebrafish as a potential model species to further elucidate the function of ADAMTS enzymes during vertebrate development. The online version of this article (doi:10.1186/s12862-015-0281-9) contains supplementary material, which is available to authorized users.
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影响因子：
1.5
作者：
Dubail, Johanne;Aramaki-Hattori, Noriko;Bader, Hannah L.;Nelson, Courtney M.;Katebi, Negin;Matuska, Brittany;Olsen, Bjorn R.;Apte, Suneel S.
通讯作者：
Apte, Suneel S.
DOI：
10.1073/pnas.48.6.1014
发表时间：
1962-01-01
影响因子：
11.1
作者：
GROSS, J;LAPIERE, CM
通讯作者：
LAPIERE, CM
影响因子：
4.8
作者：
Abbaszade, I;Liu, RQ;Burn, TC
通讯作者：
Burn, TC
影响因子：
4.4
作者：
Charlier C;Montfort J;Chabrol O;Brisard D;Nguyen T;Le Cam A;Richard-Parpaillon L;Moreews F;Pontarotti P;Uzbekova S;Chesnel F;Bobe J
通讯作者：
Bobe J
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM