Oocyte-somatic cells interactions, lessons from evolution.
Oocyte-somatic cells interactions, lessons from evolution.
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DOI：
10.1186/1471-2164-13-560
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发表时间：
2012-10-19
期刊：
影响因子：
4.4
通讯作者：
Bobe J
中科院分区：
文献类型：
--
作者：
Charlier C;Montfort J;Chabrol O;Brisard D;Nguyen T;Le Cam A;Richard-Parpaillon L;Moreews F;Pontarotti P;Uzbekova S;Chesnel F;Bobe J
尽管体细胞对卵母细胞发育能力的获得具有重要意义，但获得完全发育能力的整体机制还远未被了解，尤其是在非哺乳动物物种中。目前的工作旨在确定脊椎动物共享的来自体细胞起源的关键分子信号。通过对4种脊椎动物--一种硬骨鱼、一种两栖动物和两种哺乳动物--在发育能力获得的相似关键步骤进行平行转录分析，我们发现了大量物种特有的差异表达基因和数量惊人地多的同源基因，这些基因在三种四足动物和四种脊椎动物中表现出相似的表达谱。在进化保守的参与者中，参与获得发育能力的基因包括参与细胞能量代谢、细胞间通讯和减数分裂控制等关键过程的基因。此外，我们还报道了许多从未在脊椎动物卵巢中研究过的来自体细胞的新的分子因子。有趣的是，这些新参与者中有相当数量的人积极参与果蝇的卵子发生。我们的研究全面综述了4种脊椎动物从体细胞起源参与卵母细胞发育能力获得的进化保守机制。综上所述，我们的结果表明，尽管卵泡结构有很大的差异，但体细胞来源的一些参与卵母细胞发育能力获得的关键角色将是共同的，不仅是脊椎动物，后生动物也是如此。脊椎动物进化过程中这些机制的保守进一步强调了体细胞间隔对卵母细胞质量的重要贡献，并为进一步研究如何更好地理解好的卵子铺平了道路。
Despite the known importance of somatic cells for oocyte developmental competence acquisition, the overall mechanisms underlying the acquisition of full developmental competence are far from being understood, especially in non-mammalian species. The present work aimed at identifying key molecular signals from somatic origin that would be shared by vertebrates. Using a parallel transcriptomic analysis in 4 vertebrate species - a teleost fish, an amphibian, and two mammals - at similar key steps of developmental competence acquisition, we identified a large number of species-specific differentially expressed genes and a surprisingly high number of orthologous genes exhibiting similar expression profiles in the 3 tetrapods and in the 4 vertebrates. Among the evolutionary conserved players participating in developmental competence acquisition are genes involved in key processes such as cellular energy metabolism, cell-to-cell communications, and meiosis control. In addition, we report many novel molecular actors from somatic origin that have never been studied in the vertebrate ovary. Interestingly, a significant number of these new players actively participate in Drosophila oogenesis. Our study provides a comprehensive overview of evolutionary-conserved mechanisms from somatic origin participating in oocyte developmental competence acquisition in 4 vertebrates. Together our results indicate that despite major differences in ovarian follicular structure, some of the key players from somatic origin involved in oocyte developmental competence acquisition would be shared, not only by vertebrates, but also by metazoans. The conservation of these mechanisms during vertebrate evolution further emphasizes the important contribution of the somatic compartment to oocyte quality and paves the way for future investigations aiming at better understanding what makes a good egg.
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影响因子：
2.5
作者：
Balboula, A. Z.;Yamanaka, K.;Takahashi, M.
通讯作者：
Takahashi, M.
影响因子：
3.6
作者：
Bridgham, JT;Johnson, AL
通讯作者：
Johnson, AL
影响因子：
2.5
作者：
Gohin, Maella;Bodinier, Pascal;Bobe, Julien
通讯作者：
Bobe, Julien
影响因子：
4.8
作者：
Couse, JF;Lindzey, J;Korach, KS
通讯作者：
Korach, KS
影响因子：
2.9
作者：
HOWE, CC;OVERTON, GC;STRICKLAND, S
通讯作者：
STRICKLAND, S