Transcriptomic profile of tobacco in response to Tomato zonate spot orthotospovirus infection.
Transcriptomic profile of tobacco in response to Tomato zonate spot orthotospovirus infection.
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烟草响应番茄带斑正孢病毒感染的转录组谱
DOI：
10.1186/s12985-017-0821-6
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发表时间：
2017-08-14
期刊：
影响因子：
4.8
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Huang C;Cun Y;Yu H;Tong Z;Xiao B;Song Z;Wang B;Li Y;Liu Y
番茄带斑病毒是中国在云南、广西两省传播的蓟马正孢子病毒的优势种，造成多种农作物减产，严重威胁农民家庭的经济收入。然而，TZSV引起作物病害的详细分子机制仍不清楚。采用基于下一代测序(NGS)的转录组分析技术(RNA-seq)，研究和比较了TZSV侵染烟草后系统叶基因表达的变化，并以模拟接种为对照。用RNA-Seq对烟草转录组数据进行从头组装和分析，鉴定出135,395个非基因。共获得2102个TZSV侵染烟草的差异表达基因，其中诱导表达基因1518个，抑制表达基因584个。基因本体论分析表明，这些DEGS与多种生物学功能相关，包括代谢过程、氧化还原过程、光合作用过程、蛋白激酶活性。对这些DEGS的KEGG通路分析表明，TZSV的致病机理可能影响到初级和次生代谢、光合作用和植物与病原菌的相互作用等多个过程。我们对TZSV感染烟草中转录变化的全球调查为了解发病和症状发展的确切分子机制提供了关键信息。这是利用转录组分析研究TZSV与植物相互作用关系的首次报道。本研究的结果将有助于我们更好地理解植物对原生孢子病毒侵染反应的复杂机制。
Tomato zonate spot virus (TZSV), a dominant species of thrips-transmitted orthotospoviruses in Yunnan and Guangxi provinces in China, causes significant loss of yield in lots of crops and is a major threat to incomes of rural families. However, the detailed molecular mechanism of crop disease caused by TZSV remains obscure. Next-generation sequencing (NGS)-based transcriptome analysis (RNA-seq) was performed to investigate and compare the gene expression changes in systemic leaves of tobacco upon infection with TZSV and mock-inoculated plants as a control. De novo assembly and analysis of tobacco transcriptome data by RNA-Seq identified 135,395 unigenes. 2102 differentially expressed genes (DEGs) were obtained in tobacco with TZSV infection, among which 1518 DEGs were induced and 584 were repressed. Gene Ontology enrichment analysis revealed that these DEGs were associated with multiple biological functions, including metabolic process, oxidation-reduction process, photosynthesis process, protein kinase activity. The KEGG pathway analysis of these DEGs indicated that pathogenesis caused by TZSV may affect multiple processes including primary and secondary metabolism, photosynthesis and plant-pathogen interactions. Our global survey of transcriptional changes in TZSV infected tobacco provides crucial information into the precise molecular mechanisms underlying pathogenesis and symptom development. This is the first report on the relationships in the TZSV-plant interaction using transcriptome analysis. Findings of present study will significantly help enhance our understanding of the complicated mechanisms of plant responses to orthotospoviral infection.
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4.4
作者：
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通讯作者：
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