Intra-individual variance of the human plasma oxylipin pattern: Low inter-day variability in fasting blood samples: Versus high variability during the day
Intra-individual variance of the human plasma oxylipin pattern: Low inter-day variability in fasting blood samples: Versus high variability during the day
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人血浆氧脂素模式的个体差异：空腹血液样本的低日间变异性：与白天的高变异性相比
DOI：
10.1039/c8ay01753k
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发表时间：
2018
影响因子：
3.1
通讯作者：
Schebb NH
中科院分区：
文献类型：
--
作者：
Ostermann AI;Greupner T;Kutzner L;Hartung NM;Hahn A;Schuchardt JP;Schebb NH
几种类二十烷酸和其他氧化脂质是重要的脂质介质。血浆中的可靠定量对于评估疾病状态、药物作用和氧脂素生物学是重要的。为了监测生物学变化，生物样品中氧脂素浓度的低背景变异性对于正确解释氧脂素生物学至关重要。然而，只有很少有人知道的变化，在健康的人类subjects.ExperimentalInter-day的变化，循环oxylipins后禁食一夜，在健康的年轻男性在一个标准化或非标准化的饮食在（24至）48小时的时间间隔。在8小时的时间间隔内（包括早餐和午餐），在0，2，4，6和8小时的血液采样的标准化饮食的男性进行了研究。使用靶向代谢组学平台分析血浆中的游离氧脂素，用于定量来自不同前体的160种氧脂素。分析变异进行了评价的基础上，质量控制血浆samples.ResultsFree羟脂质在质量控制血浆样品显示低的变化（<20%，大多数分析物）。空腹血液的日间变化在相同的范围内，而显着差异，观察在一天内（日内方差）。ConclusionBased在低的个人内日间方差的浓度的自由oxlipins，我们的研究结果表明，适合空腹血浆的调查oxlipin生物学。在非空腹血浆样本中，白天的变化较高。因此，非空腹血浆样本似乎不适合评价生物学相关变化，例如由疾病或药物引起的变化。然而，它仍然有待确定，如果相同的标准化膳食的结果在可重复的调制的氧脂素配置文件允许评价餐后状态期间的氧脂素模式。
IntroductionSeveral eicosanoids and other oxylipins are important lipid mediators. Reliable quantification in plasma is important to assess the state of disease, action of drugs and the biology of oxylipins. In order to monitor biological changes, low background variability of oxylipin concentrations in biological samples is essential for proper interpretation of oxylipin biology. However, only little is known about the variation in the oxylipin profile in healthy human subjects.ExperimentalInter-day variation in circulating oxylipins after overnight fasting was investigated in healthy young men on either a standardized or non-standardized diet during a (24 to) 48 h time interval. Intra-day variance was investigated during an 8 h time interval (covering breakfast and lunch meals) in men on a standardized diet with blood sampling at 0, 2, 4, 6 and 8 hours. Free oxylipins in plasma were analyzed using a targeted metabolomics platform for the quantification of 160 oxylipins from different precursors. Analytical variation was evaluated based on quality control plasma samples.ResultsFree oxylipins in quality control plasma samples showed low variations (<20% for most analytes). Inter-day variations in fasting blood were in the same range, while significant differences were observed within the day (intra-day variance).ConclusionBased on the low intra-individual inter-day variance in concentrations of free oxylipins, our results demonstrate the suitability of fasting plasma for the investigation of oxylipin biology. In non-fasting plasma samples, the variations were high during the day. Thus, non-fasting plasma samples appear to be unsuitable to evaluate biologically relevant changes, for instance, those caused by disease or drugs. However, it remains to be determined if the same standardized meal results in reproducible modulations of the oxylipin profile allowing evaluation of the oxylipin pattern during the postprandial state.
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影响因子：
3.8
作者：
Jelic, Katarina;Hallgreen, Christine E.;Colding-Jorgensen, Morten
通讯作者：
Colding-Jorgensen, Morten
DOI：
10.1016/j.plefa.2009.05.018
发表时间：
2009-07-01
影响因子：
3
作者：
Metherel, A. H.;Armstrong, J. M.;Stark, K. D.
通讯作者：
Stark, K. D.
Vereinfachte Mikromethode zur gravimelrischen Bbestimmung des Fettgehaltes von Lebensmitteln nach Säureaufschluss (Kurzmitteilung)
DOI：
--
发表时间：
2004
期刊：
影响因子：
--
作者：
E. Schulte
通讯作者：
E. Schulte
影响因子：
4.8
作者：
Kardinaal, Alwine F. M.;van Erk, Marjan J.;Wopereis, Suzan
通讯作者：
Wopereis, Suzan
鱼油调节健康人的血液脂肪酸组成和氧脂质水平：年轻和老年男性的比较。
影响因子：
5.2
作者：
M. Zulyniak;K. Roke;C. Gerling;Samantha Logan;L. Spriet;D. Mutch
通讯作者：
D. Mutch