A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay.
A high-throughput method for assessing chemical toxicity using a Caenorhabditis elegans reproduction assay.
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DOI：
10.1016/j.taap.2010.02.014
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发表时间：
2010-06-01
影响因子：
3.8
通讯作者：
Freedman JH
中科院分区：
文献类型：
--
作者：
Boyd WA;McBride SJ;Rice JR;Snyder DW;Freedman JH
国家研究理事会概述了非哺乳动物毒理学模型的必要性，以测试大量化学品的潜在健康影响，同时减少传统动物模型的使用。线虫秀丽隐杆线虫是一个有吸引力的替代模型，因为其良好的特点和进化保守的生物学，低成本，并能够用于高通量筛选。本文介绍了一种高通量的方法来定量测定C.使用COPAS Biosort将线虫从最后的幼虫阶段（L4）暴露于化学品48小时至成年。最初，定义了可能影响生殖的暴露条件的影响。DMSO溶剂浓度≤ 1%不影响繁殖。以往的研究表明，C.秀丽线虫可能受到暴露于低pH条件的影响。在pH大于4.5时，C.线虫的繁殖没有受到影响，但是低于该pH值，后代的数量显著减少。选择氯化镉作为模型毒物，以验证自动测量与传统观察性研究的结果相当。自动测量的镉EC 50值（176-192 μM）与之前使用手动计数报告的72小时暴露的EC 50值（151 μM）相当。研究了7种试验毒物对C.线虫的繁殖与啮齿动物的死亡率高度相关，这表明该测定方法可能用于预测化学品对其他生物的潜在毒性。
The National Research Council has outlined the need for non-mammalian toxicological models to test the potential health effects of a large number of chemicals while also reducing the use of traditional animal models. The nematode Caenorhabditis elegans is an attractive alternative model because of its well-characterized and evolutionarily-conserved biology, low cost, and ability to be used in high-throughput screening. A high-throughput method is described for quantifying the reproductive capacity of C. elegans exposed to chemicals for 48 h from the last larval stage (L4) to adulthood using a COPAS Biosort. Initially, the effects of exposure conditions that could influence reproduction were defined. Concentrations of DMSO vehicle ≤ 1% did not affect reproduction. Previous studies indicated that C. elegans may be influenced by exposure to low pH conditions. At pHs greater than 4.5, C. elegans reproduction was not affected, however below this pH there was a significant decrease in the number of offspring. Cadmium chloride was chosen as a model toxicant to verify that automated measurements were comparable to those of traditional observational studies. EC50 values for cadmium for automated measurements (176-192 μM) were comparable to those previously reported for a 72-h exposure using manual counting (151 μM). The toxicity of seven test toxicants on C. elegans reproduction was highly correlative with rodent lethality suggesting that this assay may be useful in predicting the potential toxicity of chemicals in other organisms.
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影响因子：
4.7
作者：
Artal-Sanz, Marto;de Jong, Liesbeth;Tavernarakis, Nektarios
通讯作者：
Tavernarakis, Nektarios
DOI：
10.1155/asp.2005.2229
发表时间：
2005-08-11
期刊：
EURASIP JOURNAL ON APPLIED SIGNAL PROCESSING
影响因子：
--
作者：
Geng, W;Cosman, P;Schafer, WR
通讯作者：
Schafer, WR
影响因子：
3.7
作者：
Boyd WA;McBride SJ;Freedman JH
通讯作者：
Freedman JH
影响因子：
56.9
作者：
HORVITZ, HR;CHALFIE, M;EVANS, PD
通讯作者：
EVANS, PD
DOI：
10.1080/009841099157179
发表时间：
1999-12-10
影响因子：
2.6
作者：
Dhawan, R;Dusenbery, DB;Williams, PL
通讯作者：
Williams, PL