A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging.
A cell-based biosensor for real-time detection of cardiotoxicity using lensfree imaging.
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DOI：
10.1039/c1lc20098d
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发表时间：
2011-05-21
期刊：
影响因子：
6.1
通讯作者：
Khademhosseini A
中科院分区：
文献类型：
--
作者：
Kim SB;Bae H;Cha JM;Moon SJ;Dokmeci MR;Cropek DM;Khademhosseini A
一种便携式和成本效益的实时心脏毒性生物传感器的开发使用CMOS成像模块提取的商业可用的网络摄像头。检测系统由CMOS成像模块、白色LED和针孔组成。通过比较参考帧图像和实时帧图像进行实时图像处理。为了评估工程系统，测量两种不同药物，异丙肾上腺素和阿霉素对ESC衍生的心肌细胞的搏动率和搏动间变化的影响。使用检测系统得出结论，在用异丙肾上腺素和多柔比星治疗下，心跳间变异性增加。然而，跳动率增加后，加入异丙肾上腺素，但减少与阿霉素补充的文化。此外，在异丙肾上腺素治疗下，ESC衍生的心肌细胞的搏动率和搏动间变异性的响应时间比阿霉素短，尽管在测量中使用的异丙肾上腺素的量比阿霉素低三个数量级。鉴于其以简单且廉价的方式执行实时细胞监测的能力，所提出的系统可用于一系列基于细胞的生物传感应用。
A portable and cost-effective real-time cardiotoxicity biosensor was developed using a CMOS imaging module extracted from a commercially available webcam. The detection system consists of a CMOS imaging module, a white LED and a pinhole. Real-time image processing was conducted by comparing reference and live frame images. To evaluate the engineered system, the effects of two different drugs, isoprenaline and doxorubicin, on the beating rate and beat-to-beat variations of ESC-derived cardiomyocytes were measured. The detection system was used to conclude that the beat-to-beat variability increased under treatment with both isoprenaline and doxorubicin. However, the beating rates increased upon the addition of isoprenaline but decreased for cultures supplemented with doxorubicin. Moreover, the response time for both the beating rates and the beat-to-beat variability of ESC-derived cardiomyocytes under treatment of isoprenaline was shorter than doxorubicin, although the amount of isoprenaline used in the measurement was three orders of magnitude lower than that of doxorubicin. Given its ability to perform real-time cell monitoring in a simple and inexpensive manner, the proposed system may be useful for a range of cell-based biosensing applications.
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影响因子：
7.4
作者：
Force, Thomas;Kerkelae, Risto
通讯作者：
Kerkelae, Risto
影响因子：
6.1
作者：
Seo S;Su TW;Tseng DK;Erlinger A;Ozcan A
通讯作者：
Ozcan A
影响因子：
12.6
作者：
CONNOLLY, P;CLARK, P;WILKINSON, CDW
通讯作者：
WILKINSON, CDW
影响因子：
4
作者：
Khademhosseinieh, Bahar;Biener, Gabriel;Ozcan, Aydogan
通讯作者：
Ozcan, Aydogan
DOI：
10.1073/pnas.0804612105
发表时间：
2008-08-05
影响因子：
11.1
作者：
Cui, Xiquan;Lee, Lap Man;Yang, Changhuei
通讯作者：
Yang, Changhuei