Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens.
Standardization of G. mellonella Larvae to Provide Reliable and Reproducible Results in the Study of Fungal Pathogens.
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G.梅洛尼菌幼虫的标准化在真菌病原体的研究中提供可靠且可重复的结果。
DOI：
10.3390/jof4030108
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发表时间：
2018-09-06
期刊：
影响因子：
--
通讯作者：
Bates S
中科院分区：
文献类型：
--
作者：
Champion OL;Titball RW;Bates S
在过去的十年中，蜡螟幼虫已被广泛用作非哺乳动物感染模型。然而，这种感染模式的全部潜力尚未实现，受到所用幼虫质量不一和缺乏标准化程序的限制。在这里，我们审查幼虫适合研究，给药幼虫的协议，并在幼虫感染真菌病原体的疾病评分的方法。开展我们的实验工作的标准化方案的开发将允许开发高通量筛选，改变我们评估突变体和菌株面板的方式。它还将使潜在的抗菌剂在研究和开发周期的早期阶段进行体内筛选。
In the past decade, Galleria mellonella (wax moth) larvae have become widely used as a non-mammalian infection model. However, the full potential of this infection model has yet to be realised, limited by the variable quality of larvae used and the lack of standardised procedures. Here, we review larvae suitable for research, protocols for dosing larvae, and methods for scoring illness in larvae infected with fungal pathogens. The development of standardised protocols for carrying out our experimental work will allow high throughput screens to be developed, changing the way in which we evaluate panels of mutants and strains. It will also enable the in vivo screening of potential antimicrobials at an earlier stage in the research and development cycle.
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影响因子：
5.7
作者：
Dalton, James P.;Uy, Benedict;Wiles, Siouxsie
通讯作者：
Wiles, Siouxsie
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2.9
作者：
Cecilia Mesa-Arango, Ana;Forastiero, Agustina;Zaragoza, Oscar
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Zaragoza, Oscar
影响因子：
5.2
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Browne N;Heelan M;Kavanagh K
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Kavanagh K
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5.2
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Banville, Nessa;Browne, Niall;Kavanagh, Kevin
通讯作者：
Kavanagh, Kevin
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3.7
作者：
Chibebe Junior, Jose;Fuchs, Beth B.;Mylonakis, Eleftherios
通讯作者：
Mylonakis, Eleftherios