Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development
Genome-Wide Investigation of Heat Shock Transcription Factor Family in Wheat (Triticum aestivum L.) and Possible Roles in Anther Development
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小麦热激转录因子家族的全基因组研究及其在花药发育中的可能作用
DOI：
10.3390/ijms21020608
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发表时间：
2020-01
影响因子：
5.6
通讯作者：
Song Xiyue
中科院分区：
文献类型：
--
作者：
Ye Jiali;Yang Xuetong;Hu Gan;Liu Qi;Li Wei;Zhang Lingli;Song Xiyue
热激转录因子（HSFs）在植物抵抗热胁迫和调节生长发育中起着重要作用。近年来，研究表明HSFs在花药发育中发挥重要作用。因此，研究HSF家族成员及其在小麦花药中的保护作用，对小麦雄性不育育种的进一步发展具有重要意义。本研究在小麦全基因组中鉴定了61个小麦HSF基因（TaHsfs），它们不均匀地分布在21条染色体上。根据基因结构和系统发育分析，将61株TaHsfs分为3大类12个亚类。通过对14对同源异型三联体的分析，发现全基因组重复是小麦HSF基因家族扩展的主要来源，而只有极少数的TaHsfs是通过片段重复和串联重复获得的。热休克蛋白90（HSP 90），HSP 70，和另一类伴侣蛋白htpG被确定为与小麦HSFs相互作用的蛋白质。RNA-seq分析表明，TaHsfs具有明显的时期特异性和组织特异性表达模式，其中A类和B类TaHsfs对热激有反应，C类TaHsfs参与干旱调控。qRT-PCR鉴定出3个在不育和可育花药中差异表达的TaHsfA2bs，它们可能是参与花药发育的候选基因。这一全面的分析提供了新的见解TaHsfs，这将有助于理解植物育性转换的机制。
Heat shock transcription factors (HSFs) play crucial roles in resisting heat stress and regulating plant development. Recently, HSFs have been shown to play roles in anther development. Thus, investigating the HSF family members and identifying their protective roles in anthers are essential for the further development of male sterile wheat breeding. In the present study, 61 wheat HSF genes (TaHsfs) were identified in the whole wheat genome and they are unequally distributed on 21 chromosomes. According to gene structure and phylogenetic analyses, the 61 TaHsfs were classified into three categories and 12 subclasses. Genome-wide duplication was identified as the main source of the expansion of the wheat HSF gene family based on 14 pairs of homeologous triplets, whereas only a very small number of TaHsfs were derived by segmental duplication and tandem duplication. Heat shock protein 90 (HSP90), HSP70, and another class of chaperone protein called htpG were identified as proteins that interact with wheat HSFs. RNA-seq analysis indicated that TaHsfs have obvious period- and tissue-specific expression patterns, and the TaHsfs in classes A and B respond to heat shock, whereas the C class TaHsfs are involved in drought regulation. qRT-PCR identified three TaHsfA2bs with differential expression in sterile and fertile anthers, and they may be candidate genes involved in anther development. This comprehensive analysis provides novel insights into TaHsfs, and it will be useful for understanding the mechanism of plant fertility conversion.
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影响因子：
6.9
作者：
Frank G;Pressman E;Ophir R;Althan L;Shaked R;Freedman M;Shen S;Firon N
通讯作者：
Firon N
DOI：
10.1038/npg.els.0002015
发表时间：
2001-07
期刊：
--
影响因子：
--
作者：
J. Bennetzen;K. Devos
通讯作者：
J. Bennetzen;K. Devos
影响因子：
14.9
作者：
Mistry J;Finn RD;Eddy SR;Bateman A;Punta M
通讯作者：
Punta M
PlantTFDB 4.0：成为植物转录因子和调控相互作用的中心枢纽。
影响因子：
14.9
作者：
Jin J;Tian F;Yang DC;Meng YQ;Kong L;Luo J;Gao G
通讯作者：
Gao G
影响因子：
5.3
作者：
Liu Z;Xin M;Qin J;Peng H;Ni Z;Yao Y;Sun Q
通讯作者：
Sun Q