Streptococcus gordonii DL1 evades polymorphonuclear leukocyte-mediated killing via resistance to lysozyme.
Streptococcus gordonii DL1 evades polymorphonuclear leukocyte-mediated killing via resistance to lysozyme.
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DOI：
10.1371/journal.pone.0261568
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Takahashi Y
中科院分区：
文献类型：
--
作者：
Urano-Tashiro Y;Saiki K;Yamanaka Y;Ishikawa Y;Takahashi Y
戈登链球菌是感染性心内膜炎的病原菌。虽然发病机制还不清楚，链球菌和吞噬细胞之间的相互作用被认为是重要的感染性心内膜炎的发展。先前的研究表明，一些S.戈登氏菌菌株，包括DL1，在多形核白细胞（PMN）中存活，而其它菌株如SK12对PMN依赖性杀伤敏感。在这项研究中，我们评估了S。gordonii DL1和S. gordonii SK12对PMN依赖性杀伤的作用。S.当用PMNs处理时，戈登氏菌DL1显示出比SK12更高的存活率。两个S。gordonii DL1和S.戈登氏菌SK 12对低pH条件具有较强的抗性。相比S。gordonii SK 12、S.戈登氏菌DL 1对过氧化氢敏感。然而，S.戈登氏菌DL 1对供试杀菌剂尤其是溶菌酶的杀菌活性高于SK 12。此外，我们通过处理S. gordonii DL1和SK 12与PMN的结合。S. gordonii DL1对S. gordonii SK12暴露于PMNs。这些结果表明，S.戈登氏菌DL1对降解吞噬溶酶体中细菌的杀菌剂具有抗性。此外，没有分泌因子参与杀菌剂的耐药性。本研究的发现可能有助于开发由S. gordonii。
Streptococcus gordonii is an etiological bacterial agent of infective endocarditis. Although the pathogenesis mechanisms are not well understood, the interaction between streptococci and phagocytes is considered important for the development of infective endocarditis. Previous studies show that some S. gordonii strains, including DL1, survive in polymorphonuclear leukocytes (PMNs), whereas other strains such as SK12 are sensitive to PMN-dependent killing. In this study, we assessed the differences between the sensitivity of S. gordonii DL1 and S. gordonii SK12 to PMN-dependent killing. S. gordonii DL1 showed a higher survival when treated with PMNs than SK12. Both S. gordonii DL1 and S. gordonii SK12 showed high resistance to low pH condition. Compared to S. gordonii SK12, S. gordonii DL1 was sensitive to hydrogen peroxide. However, the resistance of S. gordonii DL1 to the tested bactericidal agents, especially lysozyme, was higher than that of SK12. Furthermore, we performed a bactericidal assay by treating a mixture of S. gordonii DL1 and SK12 with PMNs. S. gordonii DL1 did not enhance the survival of S. gordonii SK12 exposed to PMNs. These results indicated that S. gordonii DL1 is resistant to bactericidal agents that degrade bacteria in phagolysosomes. In addition, there was no secretory factor involved in the resistance to bactericidal agents. The findings of this study may help develop treatments for infective endocarditis caused by S. gordonii.
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