Olfactory ensheathing cells enhance Schwann cell-mediated anatomical and functional repair after sciatic nerve injury in adult rats
Olfactory ensheathing cells enhance Schwann cell-mediated anatomical and functional repair after sciatic nerve injury in adult rats
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嗅鞘细胞增强成年大鼠坐骨神经损伤后雪旺细胞介导的解剖和功能修复
DOI：
10.1016/j.expneurol.2010.08.034
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发表时间：
2011-05
影响因子：
5.3
通讯作者：
Li BC
中科院分区：
文献类型：
--
作者：
You H;Wei L;Liu Y;Oudega M;Jiao SS;Feng SN;Chen Y;Chen JM;Li BC
坐骨神经损伤会导致轴突损伤、肌肉变性和功能丧失。我们比较了雪旺细胞(SC)、嗅鞘细胞(OEC)或SC/OEC混合移植在成年大鼠坐骨神经切断后的解剖和功能恢复方面的潜力。将细胞种植到20 mm长的大孔聚(dl-丙交酯-乙交酯)酸导管中，并在坐骨神经残端之间移植。一些大鼠接受了没有细胞的导管(对照组)或自体神经移植，这是临床护理的标准。与SC移植相比，OEC移植的轴突再生少25%，而SC/OEC移植的轴突再生多28%。腓肠肌修复与SC或OEC移植相似，SC/OEC移植好35%。SC移植组的运动和感觉功能恢复及电生理结果与OEC移植组相似，比SC/OEC移植组好33%。与SC/OEC混合移植相比，自体周围神经移植轴突再生好21%，腓肠肌恢复好18%，但这些改善并未转化为功能和电生理结果的增加。结果表明，OEC对SC介导的坐骨神经修复有协同作用。数据强调了干细胞/嗅鞘细胞移植作为人工神经用于周围神经修复的前景。
Sciatic nerve injury results in axon damage, muscle degeneration, and loss of function. We compared the potential of Schwann cell (SC), olfactory ensheathing cell (OEC), or mixed SC/OEC transplants for anatomical and functional restoration after adult rat sciatic nerve transection. The cells were seeded into a 20mm long macroporous poly(dl-lactide-co-glycolide) acid conduit and grafted between the sciatic nerve stumps. Some rats received a conduit without cells (controls) or an autologous nerve graft, the clinical standard of care. Compared with SC transplants, axon regeneration was 25% less with OEC transplants but 28% more with SC/OEC transplants. Gastrocnemius muscle restoration was similar with a SC or OEC transplant and 35% better with a SC/OEC transplant. With SC transplants, motor and sensory function recovery and electrophysiological outcomes were similar as with OEC transplants and 33% better with SC/OEC transplants. Compared with the mixed SC/OEC transplants, axon regeneration was 21% better and gastrocnemius muscle restoration was 18% better with autologous peripheral nerve transplants, but these improvements did not translate into increased function and electrophysiological outcomes. Our results revealed that OEC synergistically improve SC mediated sciatic nerve repair. The data emphasized the promise of SC/OEC transplants as artificial nerves for peripheral nerve repair.
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DOI：
10.1523/jneurosci.22-15-06670.2002
发表时间：
2002-08
期刊：
The Journal of Neuroscience
影响因子：
--
作者：
T. Takami;M. Oudega;Margaret L. Bates;P. Wood;N. Kleitman;M. Bunge
通讯作者：
T. Takami;M. Oudega;Margaret L. Bates;P. Wood;N. Kleitman;M. Bunge
DOI：
10.1523/jneurosci.11-08-02433.1991
发表时间：
1991-08
期刊：
--
影响因子：
--
作者：
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通讯作者：
T. K. Morrissey;N. Kleitman;R. Bunge
DOI：
10.1523/jneurosci.23-28-09276.2003
发表时间：
2003-10
期刊：
The Journal of Neuroscience
影响因子：
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作者：
L. Jones;Dana Sajed;M. Tuszynski
通讯作者：
L. Jones;Dana Sajed;M. Tuszynski
影响因子：
2.9
作者：
Marastoni, Stefano;Ligresti, G.;Mongiat, Maurizio
通讯作者：
Mongiat, Maurizio
DOI：
10.1523/jneurosci.22-14-06083.2002
发表时间：
2002-07
期刊：
The Journal of Neuroscience
影响因子：
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作者：
G. Plant;P. Currier;Ernesto P Cuervo;Margaret L. Bates;Yelena Pressman;M. Bunge;P. Wood
通讯作者：
G. Plant;P. Currier;Ernesto P Cuervo;Margaret L. Bates;Yelena Pressman;M. Bunge;P. Wood