Regulation of baboon fetal adrenal androgen production by adrenocorticotropic hormone, prolactin, and growth hormone.
Regulation of baboon fetal adrenal androgen production by adrenocorticotropic hormone, prolactin, and growth hormone.
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促肾上腺皮质激素、催乳素和生长激素对狒狒胎儿肾上腺雄激素产生的调节。
DOI：
10.1095/biolreprod33.3.545
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发表时间：
1985
影响因子：
3.6
通讯作者：
Albrecht,ED
中科院分区：
文献类型：
--
作者：
Pepe,GJ;Albrecht,ED
促肾上腺皮质激素（ACTH）以外的因素被认为会影响灵长类动物怀孕期间胎儿肾上腺类固醇的生成。因此，我们确定了催乳素 (Prl)、生长激素 (GH)、人绒毛膜促性腺激素 (hCG) 以及 ACTH 对胶原酶分散的狒狒胎儿肾上腺细胞分泌类固醇的影响。肾上腺是从妊娠第 100-107 天（足月 = 第 184 天）剖腹产后的 7 个狒狒 (Papio anubis) 胎儿身上获取的。用细剪刀切碎组织，用0.2％胶原酶分散细胞，然后用含有青霉素/链霉素的培养基199洗涤。将细胞 (0.5 × 104) 置于含有或不含 10 nmol 羊 Prl、羊 GH 或 ACTH 或 50 nmol hCG 的 4 ml 培养基 199 中。孵育18小时（37°C）后，通过离心分离细胞，并测定分泌到培养基中的皮质醇（F）、脱氢表雄酮（DHA）和硫酸DHA（DHAS）的量。在对照组中，DHA 分泌量 [224 ± 96 ng/(24 h × 105cells)] 高于 (P< 0.05) DHAS (20 ± 12) 和 F (14 ± 12)。促肾上腺皮质激素、Prl 和 GH 刺激 (P< 0.05) DHA 分泌量为 370% ± 71%、215% ± 61%，分别为 292% ± 73%；主要由于分泌率相对较低，激素处理不会改变 DHAS 和 F 的分泌。此外，在存在或不存在 ACTH 的情况下，向培养基中添加 20 nmol 孕酮不会影响 F 的产生。这些结果表明，狒狒胎儿肾上腺在妊娠中期不利用胎盘孕酮进行 F 合成。调节狒狒胎儿肾上腺 DHA 分泌而不改变妊娠中期 F 和 DHAS 的产生，因此，我们认为妊娠中期可能存在不止一种因素调节狒狒胎儿肾上腺雄激素分泌。
Factors other than adrenocorticotropic hormone (ACTH) are thought to influence fetal adrenal steroidogenesis during primate pregnancy. Therefore, we determined the effects of prolactin (Prl), growth hormone (GH), and human chorionic gonadotropin (hCG) as well as ACTH on steroid secretion by collagenase-dispersed baboon fetal adrenal cells. Adrenal glands were obtained from seven baboon (Papio anubis) fetuses following cesarean section at Day 100–107 of gestation (term = Day 184). Tissue was minced with a fine scissors and cells were dispersed with 0.2% collagenase, then washed with Medium 199 containing penicillin/streptomycin. Cells (0.5 × 104) were placed in 4 ml Medium 199 with or without 10 nmol ovine Prl, ovine GH, or ACTH, or 50 nmol hCG. After 18 h incubation (37°C), cells were separated by centrifugation and the quantities of cortisol (F), dehydroepiandrosterone (DHA), and DHA-sulfate (DHAS) secreted into the medium were determined. In controls, DHA secretion [224 ± 96 ng/(24 h × 105cells] was greater (P< 0.05) than that of DHAS (20 ± 12) and F (14 ± 12). Adrenocorticotropic hormone, Prl, and GH stimulated (P< 0.05) DHA secretion by 370% ± 71%, 215% ± 61%, and 292% ± 73%, respectively; hCG was not effective. Due primarily to the relatively low secretion rates, DHAS and F secretion were not altered by hormonal treatment. Moreover, addition of 20 nmol progesterone to the medium in the presence or absence of ACTH did not influence F production. These findings indicate that the baboon fetal adrenal at midgestation does not utilize placental progesterone for F synthesis.We conclude that Prl, GH, and ACTH modulate baboon fetal adrenal DHA secretion without altering the production of F and DHAS at midgestation. Therefore, we suggest that ax midgestation there is probably more than one factor regulating baboon fetal adrenal androgen secretion.
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影响因子：
3.6
作者：
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DOI：
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影响因子：
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DOI：
10.1210/jcem-47-3-603
发表时间：
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期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
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