Phosphate transporters in marine phytoplankton and their viruses: cross-domain commonalities in viral-host gene exchanges.
Phosphate transporters in marine phytoplankton and their viruses: cross-domain commonalities in viral-host gene exchanges.
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DOI：
10.1111/j.1462-2920.2011.02576.x
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发表时间：
2012-01
影响因子：
5.1
通讯作者：
Worden AZ
中科院分区：
文献类型：
--
作者：
Monier A;Welsh RM;Gentemann C;Weinstock G;Sodergren E;Armbrust EV;Eisen JA;Worden AZ
磷酸盐（PO 4）是海洋环境中重要的限制性营养盐。海洋蓝细菌PSTPO 4使用高亲和力的周质磷酸盐结合蛋白PstS。最近在蓝藻病毒的基因组中也发现了pstS基因。在这里，我们分析基因编码转运蛋白的基因组中的病毒感染真核浮游植物。我们确定了无机PO 4转运蛋白编码基因的PHO 4超家族在几个病毒基因组中，沿着与其他转运蛋白编码基因。在这些病毒感染的属的基因组序列中也鉴定出病毒pho 4基因的同源物。基因组序列可从宿主属的浮游植物病毒分析，除了宿主属深球藻。通过对来自未培养的大西洋种群的靶向宏基因组进行测序，从深海球菌中回收了Pho 4。系统发育重建表明，pho 4基因从pelagophytes，haptophytes和感染病毒更密切相关的同源物prasinophytes比那些在什么，在物种水平上，被认为是更近的亲戚（如硅藻）。我们还在海洋宏基因组中鉴定了PHO 4超家族成员，包括来自太平洋的新宏基因组。环境序列主要由浮游植物、附着植物、绿藻、病毒和细菌组成。分析表明，海洋病毒与真核和细菌宿主之间发生了多个独立的pho 4基因转移事件。此外，pho 4基因在感染海洋真核生物但不感染陆地宿主的病毒的可用基因组中被鉴定。海洋宿主-病毒基因交换的共性表明，操纵宿主-PO 4摄取是海洋系统中病毒增殖的重要适应。我们的研究结果表明，PO 4-可用性可能不会作为一个简单的自下而上的控制海洋浮游植物。
Phosphate (PO4) is an important limiting nutrient in marine environments. Marine cyanobacteria scavenge PO4 using the high-affinity periplasmic phosphate binding protein PstS. The pstS gene has recently been identified in genomes of cyanobacterial viruses as well. Here, we analyse genes encoding transporters in genomes from viruses that infect eukaryotic phytoplankton. We identified inorganic PO4 transporter-encoding genes from the PHO4 superfamily in several virus genomes, along with other transporter-encoding genes. Homologues of the viral pho4 genes were also identified in genome sequences from the genera that these viruses infect. Genome sequences were available from host genera of all the phytoplankton viruses analysed except the host genus Bathycoccus. Pho4 was recovered from Bathycoccus by sequencing a targeted metagenome from an uncultured Atlantic Ocean population. Phylogenetic reconstruction showed that pho4 genes from pelagophytes, haptophytes and infecting viruses were more closely related to homologues in prasinophytes than to those in what, at the species level, are considered to be closer relatives (e.g. diatoms). We also identified PHO4 superfamily members in ocean metagenomes, including new metagenomes from the Pacific Ocean. The environmental sequences grouped with pelagophytes, haptophytes, prasinophytes and viruses as well as bacteria. The analyses suggest that multiple independent pho4 gene transfer events have occurred between marine viruses and both eukaryotic and bacterial hosts. Additionally, pho4 genes were identified in available genomes from viruses that infect marine eukaryotes but not those that infect terrestrial hosts. Commonalities in marine host-virus gene exchanges indicate that manipulation of host-PO4 uptake is an important adaptation for viral proliferation in marine systems. Our findings suggest that PO4-availability may not serve as a simple bottom-up control of marine phytoplankton.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
56.9
作者：
Armbrust, EV;Berges, JA;Rokhsar, DS
通讯作者：
Rokhsar, DS
影响因子：
2.8
作者：
Díaz, M;Esteban, A;Santamaría, RI
通讯作者：
Santamaría, RI
DOI：
10.1073/pnas.1001665107
发表时间：
2010-08-17
影响因子：
11.1
作者：
Cuvelier, Marie L.;Allen, Andrew E.;Worden, Alexandra Z.
通讯作者：
Worden, Alexandra Z.
DOI：
10.1016/j.bbrc.2004.11.125
发表时间：
2005-01-28
影响因子：
3.1
作者：
Chen, J;Cassar, SC;Gopalakrishnan, M
通讯作者：
Gopalakrishnan, M