Maintenance and distribution of epithelial stem/progenitor cells after corneal reconstruction using oral mucosal epithelial cell sheets.
Maintenance and distribution of epithelial stem/progenitor cells after corneal reconstruction using oral mucosal epithelial cell sheets.
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DOI：
10.1371/journal.pone.0110987
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Nishida K
中科院分区：
文献类型：
--
作者：
Soma T;Hayashi R;Sugiyama H;Tsujikawa M;Kanayama S;Oie Y;Nishida K
我们在大鼠模型中使用组织工程口腔粘膜细胞片评估了角膜重建后上皮干/祖细胞的维持和分布。从绿色荧光蛋白 (GFP) 大鼠身上切下口腔粘膜活检标本，并用 Dispase II 进行酶处理。这些细胞与经丝裂霉素 C 处理的 NIH/3T3 细胞一起在插入物上培养，并收获所得的细胞片。这些来自 GFP 大鼠的组织工程细胞片被移植到裸鼠角膜缘干细胞缺陷模型的眼睛上。手术后八周，眼表完全被带有 GFP 阳性细胞的上皮覆盖。移植的角膜在基底层表达p63，在所有上皮层表达K14。分离重建角膜中央和周边区域的上皮细胞进行集落形成实验，结果显示角膜重建后8周，周边上皮细胞的集落形成效率显着高于中央上皮细胞。因此，在该大鼠模型中，使用口腔粘膜上皮细胞片进行角膜重建后，周边角膜可以比中央角膜保留更多的干/祖细胞。
We assessed the maintenance and distribution of epithelial stem/progenitor cells after corneal reconstruction using tissue-engineered oral mucosal cell sheets in a rat model. Oral mucosal biopsy specimens were excised from green fluorescent protein (GFP) rats and enzymatically treated with Dispase II. These cells were cultured on inserts with mitomycin C-treated NIH/3T3 cells, and the resulting cell sheets were harvested. These tissue-engineered cell sheets from GFP rats were transplanted onto the eyes of a nude rat limbal stem cell deficiency model. Eight weeks after surgery, ocular surfaces were completely covered by the epithelium with GFP-positive cells. Transplanted corneas expressed p63 in the basal layers and K14 in all epithelial layers. Epithelial cells harvested from the central and peripheral areas of reconstructed corneas were isolated for a colony-forming assay, which showed that the colony-forming efficiency of the peripheral epithelial cells was significantly higher than that of the central epithelial cells 8 weeks after corneal reconstruction. Thus, in this rat model, the peripheral cornea could maintain more stem/progenitor cells than the central cornea after corneal reconstruction using oral mucosal epithelial cell sheets.
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影响因子：
4.2
作者：
Santos, MS;Gomes, JAP;Belfort, R
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