Development of tri-nucleotide microsatellite markers from Crassostrea hongkongensis using enriched genomic libraries and cross-species amplification in two closely related species
Development of tri-nucleotide microsatellite markers from Crassostrea hongkongensis using enriched genomic libraries and cross-species amplification in two closely related species
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利用富集基因组文库和两个密切相关的物种的跨物种扩增，开发香港巨牡蛎的三核苷酸微卫星标记
DOI：
10.1016/j.aqrep.2021.100592
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发表时间：
2021-03
影响因子：
3.7
通讯作者：
Ziniu Yu
中科院分区：
文献类型：
--
作者：
Haitao Ma;Yuehuan Zhang;Zhiming Xiang;Yang Zhang;Yanping Qin;Ziniu Yu
使用富集方案来分离和表征香港牡蛎（Crassostrea hongkongensis）的三核苷酸微卫星标记，香港牡蛎是中国南部沿海地区经济上重要且广泛养殖的牡蛎物种。对来自野生种群的 32 个个体的 30 个三核苷酸微卫星位点进行了评估。等位基因的数量从2到12个不等，平均每个位点有7.63个等位基因。观察到的杂合度值和预期杂合度值的范围分别为 0.031 至 0.844（平均 0.579）和 0.062 至 0.881（平均 0.665）。 Bonferroni 校正后，有 6 个位点显着偏离 Hardy-Weinberg 平衡。 BLAST分析表明，从C.hongkongensis基因组中随机开发的三核苷酸微卫星标记与功能基因的相关性高于二核苷酸标记。交叉扩增 C.阿里肯西斯和 C.进行了 gigas 分析，21 个位点在 C.之间显示出合理的多态性。香港草和C. ariakensis 之间的 15 个位点显示出合理的多态性。香港草和C.千兆。本研究中鉴定的三核苷酸微卫星标记比先前鉴定的二核苷酸标记在未来用于基因作图、分子标记辅助选择和比较连锁作图方面具有更大的潜力。此外，我们还发现了两个微卫星位点，可以有效识别这三种牡蛎的纯种和杂交后代。
An enrichment protocol was used to isolate and characterize tri-nucleotide microsatellite markers inCrassostrea hongkongensis, an economically important and extensively cultured oyster species along the southern coastal areas of China. Thirty tri-nucleotide microsatellite loci were assessed in 32 individuals from a wild population. The number of alleles ranged from 2 to 12, averaging 7.63 alleles per locus. The observed and expected heterozygosity values ranged from 0.031 to 0.844 (averaging 0.579) and 0.062 to 0.881 (averaging 0.665), respectively. Six loci significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction. BLAST analysis showed that the tri-nucleotide microsatellite markers randomly developed from the genome ofC.hongkongensishave a higher correlation with functional genes than di-nucleotide markers. Cross-amplification inC. ariakensisandC. gigaswas performed and 21 loci showed reasonable polymorphism betweenC. hongkongensisandC. ariakensis, while 15 loci showed reasonable polymorphism betweenC. hongkongensisandC. gigas. The tri-nucleotide microsatellite markers identified in this study have greater potential for future use in gene mapping, molecular-marker assisted selection and comparative linkage mapping than previously identified di-nucleotide markers. Additionally, we found two microsatellite loci that can effectively identify the purebred and hybrid offspring of these three oyster species.
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影响因子：
2.4
作者：
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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影响因子：
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作者：
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