Disease outcome and cytokine responses in cats immunized with an avirulent feline infectious peritonitis virus (FIPV)-UCD1 and challenge-exposed with virulent FIPV-UCD8.
Disease outcome and cytokine responses in cats immunized with an avirulent feline infectious peritonitis virus (FIPV)-UCD1 and challenge-exposed with virulent FIPV-UCD8.
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DOI：
10.1016/j.jfms.2003.08.009
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发表时间：
2004-04
影响因子：
1.7
通讯作者：
Pedersen NC
中科院分区：
文献类型：
--
作者：
Kiss I;Poland AM;Pedersen NC
用猫传染性腹膜炎病毒（FIPV）-UCD 1的无毒毒株免疫8只猫，然后攻击暴露于高毒力猫传代株（FIPV-UCD 8）。在整个实验过程中测量外周血单核细胞（PBMC）中的Th 1和Th 2细胞因子谱。免疫后，实验猫中无明显的FIP临床体征。攻击后，免疫猫表现出四种临床结果之一：（1）经典的渗出性FIP;（2）加速FIP;（3）非渗出性FIP，或（4）对攻击的抗性。免疫后仅观察到轻微的细胞因子变化，然而，在攻击-暴露后发生了几种细胞因子变化。最值得注意的变化是肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）水平。我们的初步研究结果表明，针对FIP的免疫与TNF-α和IFN-γ应答失衡相关，高TNF-α/低IFN-γ mRNA应答有利于疾病，低TNF-α/高IFN-γ mRNA应答指示免疫。
Eight cats were immunized with an avirulent strain of feline infectious peritonitis virus (FIPV)-UCD1, then challenge-exposed to a highly virulent cat passaged strain (FIPV-UCD8). Th1 and Th2 cytokine profiles in the peripheral blood mononuclear cells (PBMCs) were measured throughout in the experiment. No clinical signs of FIP were evident in the experimental cats after immunization. After challenge, the immunized cats demonstrated one of four clinical outcomes: (1) classical effusive FIP; (2) accelerated FIP; (3) non-effusive FIP, or (4) resistance to challenge. Only minor cytokine changes were observed following immunization, however, several cytokine changes occurred following challenge-exposure. The most noteworthy changes were in tumor necrosis factor-alpha (TNF-α) and interferon gamma (IFN-γ) levels. Our preliminary findings suggest that immunity against FIP is associated with TNF-α and IFN-γ response imbalance, with high TNF-α/low IFN-γ mRNA responses favouring disease and low TNF-α/high IFN-γ mRNA responses being indicative of immunity.
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