Circuit models and experimental noise measurements of micropipette amplifiers for extracellular neural recordings from live animals.
Circuit models and experimental noise measurements of micropipette amplifiers for extracellular neural recordings from live animals.
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DOI：
10.1155/2014/135026
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发表时间：
2014
影响因子：
--
通讯作者：
Lei TC
中科院分区：
文献类型：
--
作者：
Chen CH;Pun SH;Mak PU;Vai MI;Klug A;Lei TC
玻璃微量移液器广泛用于记录活动物细胞外单个神经元或神经元簇的神经活动。然而，到目前为止，还没有全面的研究与玻璃微量移液器的细胞外记录的噪音。这项工作的目的是评估影响细胞外记录的各种噪声源，并创建模型系统，在该系统中，可以测试新型微管神经放大器的设计。建立了玻璃微吸管的等效电路及其噪声模型，准确地描述了细胞外记录中的各种噪声源。测量计划，使用死亡的脑组织，以及细胞外记录神经元在下丘，一个听觉脑核的麻醉沙鼠，被用来表征噪声性能和放大效率的建议微管神经放大器。根据我们的模型，影响信噪比的主要噪声源是神经放大器的本征噪声和来自分布移液管电阻的热噪声。这两种类型的噪声进行了计算和测量，并被证明是在体内实验的背景噪声的主要来源。
Glass micropipettes are widely used to record neural activity from single neurons or clusters of neurons extracellularly in live animals. However, to date, there has been no comprehensive study of noise in extracellular recordings with glass micropipettes. The purpose of this work was to assess various noise sources that affect extracellular recordings and to create model systems in which novel micropipette neural amplifier designs can be tested. An equivalent circuit of the glass micropipette and the noise model of this circuit, which accurately describe the various noise sources involved in extracellular recordings, have been developed. Measurement schemes using dead brain tissue as well as extracellular recordings from neurons in the inferior colliculus, an auditory brain nucleus of an anesthetized gerbil, were used to characterize noise performance and amplification efficacy of the proposed micropipette neural amplifier. According to our model, the major noise sources which influence the signal to noise ratio are the intrinsic noise of the neural amplifier and the thermal noise from distributed pipette resistance. These two types of noise were calculated and measured and were shown to be the dominating sources of background noise for in vivo experiments.
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影响因子：
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DOI：
10.1109/tnsre.2009.2021607
发表时间：
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影响因子：
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