Antiserum against tumor necrosis factor enhances lipopolysaccharide fever in rats.
Antiserum against tumor necrosis factor enhances lipopolysaccharide fever in rats.
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抗肿瘤坏死因子抗血清增强大鼠脂多糖发热。
DOI：
10.1152/ajpregu.1990.258.2.r332
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kluger,MJ
中科院分区：
文献类型：
--
作者：
Long,NC;Kunkel,SL;Vander,AJ;Kluger,MJ
通过比较腹腔内和肌肉内注射10 μ g/kg脂多糖（LPS）至雄性Sprague-Dawley大鼠后的发热和血浆TNF水平，以及通过使用TNF抗血清中和内源性TNF，研究了肿瘤坏死因子（TNF，cachectin）（一种假定的内源性热原）的作用。腹腔注射LPS引起双相发热，持续约6.5小时。这些大鼠中的TNF水平在1小时达到峰值657 +/-222 U/ml，然后在第4小时下降至几乎检测不到的水平。肌内注射的动物表现出较低的阶段性发热和较低的持续TNF水平（1 h时为40 +/-10 U/ml，4 h时为18 +/-11 U/ml）。在第二项研究中，在肌内注射10微克/千克LPS之前2小时腹膜内注射已显示出中和大鼠TNF的抗血清。对照组大鼠在注射LPS前注射正常兔血清。在注射LPS后的第二个小时内，接受抗血清的动物出现发热，其倾向于低于注射对照血清的大鼠中观察到的发热（0.33 +/-0.06对0.58 +/-0.1），尽管该差异不显著。然而，在LPS后第3至第8小时，注射抗血清的大鼠的平均体温显著高于对照组大鼠（1.62 +/-0.11 vs. 1.07 +/-0.09; P = 0.0005）。（250字处删节）
The role of tumor necrosis factor (TNF, cachectin), a putative endogenous pyrogen, was investigated by comparing fever and plasma TNF levels after the intraperitoneal and intramuscular injection of 10 micrograms/kg lipopolysaccharide (LPS) into male Sprague-Dawley rats and by neutralization of endogenous TNF using TNF antiserum. An intraperitoneal injection of LPS caused a biphasic fever that lasted approximately 6.5 h. TNF levels in these rats peaked at 657 +/- 222 U/ml at 1 h then declined to virtually undetectable levels by the fourth hour. The intramuscularly injected animals showed a lower monophasic fever and low sustained TNF levels (40 +/- 10 U/ml at 1 h, 18 +/- 11 U/ml at 4 h). In a second study, an antiserum that had been shown to neutralize rat TNF was injected intraperitoneally 2 h before the intramuscular injection of 10 micrograms/kg LPS. Control rats were injected with normal rabbit serum before LPS. During the second hour after the injection of LPS, the animals that received the antiserum developed fevers that tended to be lower than those seen in the rats that were injected with control serum (0.33 +/- 0.06 vs. 0.58 +/- 0.1), although this difference was not significant. However, during the third through eighth hours after LPS, the antiserum-injected rats had mean body temperatures that were significantly higher than those of the control rats (1.62 +/- 0.11 vs. 1.07 +/- 0.09; P = 0.0005).(ABSTRACT TRUNCATED AT 250 WORDS)
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DOI：
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发表时间：
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期刊：
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作者：
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