Expression Levels of Detoxification Enzyme Genes from Dendroctonus armandi (Coleoptera: Curculionidae) Fed on a Solid Diet Containing Pine Phloem and Terpenoids.
Expression Levels of Detoxification Enzyme Genes from Dendroctonus armandi (Coleoptera: Curculionidae) Fed on a Solid Diet Containing Pine Phloem and Terpenoids.
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华山松大小蠹取食含松树韧皮部和萜类化合物固体饲料后脱磷酶基因的表达水平
DOI：
10.3390/insects12100926
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发表时间：
2021-10-11
期刊：
影响因子：
3
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Dai L;Gao H;Chen H
The bark beetle is the most well-known pest in coniferous trees worldwide. These insects only leave the host pine bark when they disperse to locate a new host. Determining how Dendroctonus armandi overcome the trees’ terpene-based defense systems has been the key problem in the study of bark beetles. Therefore, the aim of this study was to discover the molecular mechanism of insect detoxification enzymes’ ability to confer resistance to terpenes. For this purpose, the genes of cytochrome P450s, glutathione S-transferases, and carboxylesterases were studied in beetles given diets containing terpenes. The results suggest that beetles express different genes in response to terpenoids, and the responses of multiple detoxifying enzymes indicate these insects’ adaption to their chemical environment. Bark beetles overcome the toxic terpenoids produced by pine trees by both detoxifying and converting them into a pheromone system. Detoxification enzymes such as cytochrome P450s, glutathione S-transferases, and carboxylesterases are involved in the ability of Dendroctonus armandi to adapt to its chemical environment. Ten genes from these three major classes of detoxification enzymes were selected to study how these enzymes help D. armandi to respond to the host defenses. The expression profile of these detoxification enzyme genes was observed in adult beetles after feeding on different types of diet. Significant differences were observed between two types of seminatural diet containing the phloem of pines, and a purely artificial diet containing five monoterpenes ((−)-α-pinene, (−)-β-pinene, (+)-3-carene, (±)-limonene, and turpentine oil) also caused differential transcript levels in the detoxification enzyme genes. The results suggest that monoterpenes enter the beetles through different routes (i.e., respiratory and digestive systems) and cause the expression of different genes in response, which might be involved in pheromone metabolism. In addition, the xenobiotic metabolism in bark beetles should be considered as a system comprising multiple detoxifying enzymes.
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影响因子：
12.3
作者：
Keeling CI;Yuen MM;Liao NY;Docking TR;Chan SK;Taylor GA;Palmquist DL;Jackman SD;Nguyen A;Li M;Henderson H;Janes JK;Zhao Y;Pandoh P;Moore R;Sperling FA;Huber DP;Birol I;Jones SJ;Bohlmann J
通讯作者：
Bohlmann J
影响因子：
2.3
作者：
Fernanda Lopez, Maria;Cano-Ramirez, Caudia;Zuniga, Gerardo
通讯作者：
Zuniga, Gerardo
影响因子：
2.1
作者：
Gunasekaran, K.;Muthukumaravel, S.;Jambulingam, P.
通讯作者：
Jambulingam, P.
影响因子：
3.8
作者：
Dai, Lulu;Ma, Mingyuan;Chen, Hui
通讯作者：
Chen, Hui
影响因子：
4.1
作者：
Dai, Lulu;Ma, Junning;Chen, Hui
通讯作者：
Chen, Hui