Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest.
Draft genome of the mountain pine beetle, Dendroctonus ponderosae Hopkins, a major forest pest.
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DOI：
10.1186/gb-2013-14-3-r27
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发表时间：
2013-03-27
期刊：
影响因子：
12.3
通讯作者：
Bohlmann J
中科院分区：
文献类型：
--
作者：
Keeling CI;Yuen MM;Liao NY;Docking TR;Chan SK;Taylor GA;Palmquist DL;Jackman SD;Nguyen A;Li M;Henderson H;Janes JK;Zhao Y;Pandoh P;Moore R;Sperling FA;Huber DP;Birol I;Jones SJ;Bohlmann J
美国西部松大小蠹（Dendroctonus ponderosae霍普金斯）是北美西部松林最严重的害虫。最近的一次爆发摧毁了1 500多万公顷的松树林，对森林健康造成重大环境影响，对林业造成经济影响。疫情的爆发部分是由气候变化引起的，并将通过腐烂的森林增加碳排放。我们为山松甲虫开发了一个基因组序列资源，以更好地了解这种昆虫生物学的独特方面。从头基因组序列草案由来自单个野外收集的雄性蛹的配对末端短读段序列组装，并使用来自汇集的野外收集的蛹的配对短读段基因组序列、来自成虫甲虫组织的mRNA的配对末端短插入全转录组鸟枪测序读段和来自不同生命阶段的配对末端桑格EST序列构建。我们描述了细胞色素P450，谷胱甘肽S-转移酶，和植物细胞壁降解酶基因家族的重要生存的山松甲虫在其恶劣和营养不良的宿主环境，并检查全基因组的单核苷酸多态性变化。水平转移的细菌蔗糖-6-磷酸水解酶在基因组中是显而易见的，其组织特异性转录表明这种甲虫的功能作用。尽管鞘翅目是最大的昆虫目，有超过400，000种描述的物种，包括许多农业和森林害虫物种，但这只是鞘翅目中报告的第二个基因组序列，并将为鞘翅目和其他昆虫提供重要的资源。
The mountain pine beetle, Dendroctonus ponderosae Hopkins, is the most serious insect pest of western North American pine forests. A recent outbreak destroyed more than 15 million hectares of pine forests, with major environmental effects on forest health, and economic effects on the forest industry. The outbreak has in part been driven by climate change, and will contribute to increased carbon emissions through decaying forests. We developed a genome sequence resource for the mountain pine beetle to better understand the unique aspects of this insect's biology. A draft de novo genome sequence was assembled from paired-end, short-read sequences from an individual field-collected male pupa, and scaffolded using mate-paired, short-read genomic sequences from pooled field-collected pupae, paired-end short-insert whole-transcriptome shotgun sequencing reads of mRNA from adult beetle tissues, and paired-end Sanger EST sequences from various life stages. We describe the cytochrome P450, glutathione S-transferase, and plant cell wall-degrading enzyme gene families important to the survival of the mountain pine beetle in its harsh and nutrient-poor host environment, and examine genome-wide single-nucleotide polymorphism variation. A horizontally transferred bacterial sucrose-6-phosphate hydrolase was evident in the genome, and its tissue-specific transcription suggests a functional role for this beetle. Despite Coleoptera being the largest insect order with over 400,000 described species, including many agricultural and forest pest species, this is only the second genome sequence reported in Coleoptera, and will provide an important resource for the Curculionoidea and other insects.
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影响因子：
4.4
作者：
Cornman SR;Schatz MC;Johnston SJ;Chen YP;Pettis J;Hunt G;Bourgeois L;Elsik C;Anderson D;Grozinger CM;Evans JD
通讯作者：
Evans JD
影响因子：
4.4
作者：
Andersson MN;Grosse-Wilde E;Keeling CI;Bengtsson JM;Yuen MM;Li M;Hillbur Y;Bohlmann J;Hansson BS;Schlyter F
通讯作者：
Schlyter F
DOI：
10.1073/pnas.1011289108
发表时间：
2011-02-08
影响因子：
11.1
作者：
DiGuistini, Scott;Wang, Ye;Breuil, Colette
通讯作者：
Breuil, Colette
影响因子：
3.9
作者：
Feyereisen, R.
通讯作者：
Feyereisen, R.
影响因子：
7.7
作者：
Davis, C. S.;Mock, K. E.;Cooke, J. E. K.
通讯作者：
Cooke, J. E. K.