Neuropeptide W has cell phenotype-specific effects on the excitability of different subpopulations of paraventricular nucleus neurones.
Neuropeptide W has cell phenotype-specific effects on the excitability of different subpopulations of paraventricular nucleus neurones.
复制标题
DOI：
10.1111/j.1365-2826.2009.01904.x
复制
发表时间：
2009-10
影响因子：
3.2
通讯作者：
Ferguson AV
中科院分区：
文献类型：
--
作者：
Price CJ;Samson WK;Ferguson AV
在啮齿类动物中施用神经肽 NPW 和 NPB 已被证明可以影响多种自主神经和神经内分泌系统的活动。室旁核是下丘脑中主要的自主神经和神经内分泌整合位点，该核内的神经元表达这些配体的受体，即神经肽 B/W 受体 1 (NPBWR1)。因此，我们使用全细胞膜片钳记录结合单细胞 RT-PCR 来检查神经肽 W-23 (NPW-23) 对已识别的室旁核神经元兴奋性的影响。催产素、加压素和促甲状腺素释放激素神经元均被发现对 10 nM NPW-23 有反应，尽管在每个细胞组中均观察到去极化和超极化效应。相反，促肾上腺皮质激素释放激素细胞不受影响。使用电生理指纹将化学表型细胞群进一步细分为大细胞、神经内分泌或前自主神经元，结果表明，投射到髓质和脊髓目标的神经元主要受到 NPW-23 的抑制，而投射到正中隆起或神经叶的神经元则显示出几乎相同数量的去极化和超极化细胞。室旁核神经元的特定表型群体显示出 NPW 诱导的对兴奋性的影响，这增强了 NPB/NPW 神经肽系统作为自主神经功能调节器的重要性。
Administration of the neuropeptides NPW and NPB in rodents has been shown to influence the activity of a variety of autonomic and neuroendocrine systems. The paraventricular nucleus is a major autonomic and neuroendocrine integration site in the hypothalamus and neurons within this nucleus express the receptor for these ligands, neuropeptide B/W receptor 1 (NPBWR1). Therefore we used whole cell patch clamp recordings coupled with single cell RT-PCR to examine the effects of neuropeptide W-23 (NPW-23) on the excitability of identified paraventricular nucleus neurons. Oxytocin, vasopressin and thyrotropin releasing hormone neurons were all found to be responsive to 10 nM NPW-23, although both depolarizing and hyperpolarizing effects were observed in each of these cell groups. In contrast corticotropin releasing hormone cells were unaffected. Further subdivision of chemically phenotyped cell groups into magnocellular, neuroendocrine or pre-autonomic neurons, using their electrophysiological fingerprints, revealed that neurons projecting to medullary and spinal targets were predominantly inhibited by NPW-23 while those that projected to median eminence or neural lobe showed nearly equivalent numbers of depolarizing and hyperpolarizing cells. The demonstration of particular phenotypic populations of paraventricular nucleus neurons showing NPW-induced effects on excitability reinforces the importance of the NPB/NPW neuropeptide system as a regulator of autonomic function.
登录
查看更多内容
DOI：
10.1152/ajpregu.00604.2003
发表时间：
2004-07-01
影响因子：
2.8
作者：
Blevins, JE;Schwartz, MW;Baskin, DG
通讯作者：
Baskin, DG
影响因子：
2.5
作者：
Jackson, Valerie R.;Lin, Steven H.;Civelli, Olivier
通讯作者：
Civelli, Olivier
影响因子：
3.2
作者：
Samson, WK;Baker, JR;Taylor, MM
通讯作者：
Taylor, MM
影响因子：
4
作者：
Kawasaki, Makoto;Onaka, Tatsushi;Ueta, Yoichi
通讯作者：
Ueta, Yoichi
DOI：
10.1073/pnas.0837789100
发表时间：
2003-05-13
影响因子：
11.1
作者：
Tanaka, H;Yoshida, T;Yanagisawa, M
通讯作者：
Yanagisawa, M