Spectrofluorimetric determination of human serum albumin using terbium-danofloxacin probe.
Spectrofluorimetric determination of human serum albumin using terbium-danofloxacin probe.
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DOI：
10.1100/2012/940541
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发表时间：
2012
影响因子：
--
通讯作者：
Jouyban A
中科院分区：
文献类型：
--
作者：
Ramezani AM;Manzoori JL;Amjadi M;Jouyban A
建立了以铽-达氟沙星（Tb 3 +-Dano）为荧光探针测定人血清白蛋白（HSA）和牛血清白蛋白（BSA）的荧光分析方法。这些蛋白质显著增强Tb 3 +-Dano复合物在545 nm处的荧光强度，并且增强的Tb 3 +-Dano荧光强度与蛋白质（HSA和BSA）的浓度成正比。在pH = 7.8，[Tb 3 +] = 8.5 × 10 - 5 mol L-1，[Dano] = 1.5 × 10 - 4 mol L-1时，HSA的最大响应值为1.5 × 10 - 4 mol L-1。BSA、HSA标准品溶液和HSA血浆样品的校准图分别在0.2 × 10−6 − 1.3 × 10−6 mol L−1、0.2 × 10−6 − 1.4 × 10−6 mol L−1和0.2 × 10−6 − 1 × 10−6 mol L−1范围内呈线性。BSA、HSA和HSA血浆样品的检测限（S/N = 3）分别为8.7 × 10−8 mol L−1、6.2 × 10−8 mol L−1和8.1 × 10−8 mol L−1。使用大量真实的生物血浆样品检查了该方法的适用性，并与紫外光谱参考方法进行了比较。结果表明，该方法可作为生物样品中白蛋白测定的一种简便、实用、灵敏的替代方法。
A spectrofluorimetric method is proposed for the determination of human serum albumin (HSA) and bovine serum albumin (BSA) using terbium-danofloxacin (Tb3+-Dano) as a fluorescent probe. These proteins remarkably enhance the fluorescence intensity of the Tb3+-Dano complex at 545 nm, and the enhanced fluorescence intensity of Tb3+-Dano is proportional to the concentration of proteins (HSA and BSA). Optimum conditions for the determination of HSA were investigated and found that the maximum response was observed at: pH = 7.8, [Tb3+] = 8.5 × 10−5 mol L−1, [Dano] = 1.5 × 10−4 mol L−1. The calibration graphs for standard solutions of BSA, HSA, and plasma samples of HSA were linear in the range of 0.2 × 10−6 − 1.3 × 10−6 mol L−1, 0.2 × 10−6 − 1.4 × 10−6 mol L−1, and 0.2 × 10−6 − 1 × 10−6 mol L−1, respectively. The detection limits (S/N = 3) for BSA, HSA, and plasma sample of HSA were 8.7 × 10−8 mol L−1, 6.2 × 10−8 mol L−1, and 8.1 × 10−8 mol L−1, respectively. The applicability of the method was checked using a number of real biological plasma samples and was compared with the UV spectrometric reference method. The results was showed that the method could be regarded as a simple, practical, and sensitive alternative method for determination of albumin in biological samples.
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影响因子：
4
作者：
HARA, T;TSUKAGOSHI, K;YOSHIDA, T
通讯作者：
YOSHIDA, T
影响因子：
6.2
作者：
Huang, CZ;Li, YF;Feng, P
通讯作者：
Feng, P
影响因子：
6.1
作者：
Jia, RP;Dong, LJ;Hu, ZD
通讯作者：
Hu, ZD
使用铽 (III)-2-噻吩酰基三氟丙酮-十二烷基苯磺酸钠-蛋白质系统荧光测定蛋白质
影响因子：
3.6
作者：
Jia, Zhen;Yang, Jinghe;Liu, Shufang
通讯作者：
Liu, Shufang
影响因子：
2.9
作者：
OHSAWA, K;EBATA, N
通讯作者：
EBATA, N