C60 secondary ion mass spectrometry with a hybrid-quadrupole orthogonal time-of-flight mass spectrometer.
C60 secondary ion mass spectrometry with a hybrid-quadrupole orthogonal time-of-flight mass spectrometer.
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DOI：
10.1021/ac801712s
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发表时间：
2008-11-01
影响因子：
7.4
通讯作者：
Loboda, A. V.
中科院分区：
文献类型：
--
作者：
Carado, Anthony;Passarelli, M. K.;Kozole, Joseph;Wingate, J. E.;Winograd, Nicholas;Loboda, A. V.
采用C60簇离子源，设计了一种适合MALDI和电喷雾电离的混合四极正交飞行时间质谱仪。该配置显示出许多特性，可以提高传统的飞行时间二次离子质谱（SIMS）实验的性能，用于分析复杂的有机材料，并可能用于化学成像。具体来说，主离子束作为连续离子束而不是脉冲离子束运行，导致对目标的离子影响高达4个数量级。将二次离子在极低电压下提取到8毫升氮气中，用于碰撞聚焦和冷却。这种提取配置被证明可以产生二次离子，这些二次离子会迅速失去其产生机制的记忆，从而产生与SIMS和MALDI相同的串联质谱。通过离子捕获的实现，表明萃取效率与传统的TOF-SIMS机器相当。给出了各种底物的例子，这些底物的质量分辨率为12,000-15,600，无机化合物的质量范围为m/z 40,000。初步的化学作图实验表明，随着灵敏度的增加，在MS/MS操作模式下成像是直截了当的。一般来说，MALDI和SIMS的结合为每种技术增加了功能，为已经存在于大量实验室中的TOF-SIMS实验提供了一个强大的平台。
A hybrid quadrupole orthogonal time-of-flight mass spectrometer optimized for MALDI and electrospray ionization has been equipped with a C60 cluster ion source. This configuration is shown to exhibit a number of characteristics that improve the performance of traditional time-of-flight secondary ion mass spectrometry (SIMS) experiments for the analysis of complex organic materials, and potentially, for chemical imaging. Specifically, the primary ion beam is operated as a continuous rather than a pulsed beam, resulting in up to 4 orders of magnitude greater ion fluence on the target. The secondary ions are extracted at very low voltage into 8 millitorr of N2 gas introduced for collisional focusing and cooling purposes. This extraction configuration is shown to yield secondary ions that rapidly lose memory of the mechanism of their birth, yielding tandem mass spectra that are identical for SIMS and MALDI. With implementation of ion trapping, the extraction efficiency is shown to be equivalent to that found in traditional TOF-SIMS machines. Examples are given, for a variety of substrates that illustrate mass resolution of 12,000–15,600 with mass range for inorganic compounds to m/z 40,000. Preliminary chemical mapping experiments show that with added sensitivity, imaging in the MS/MS mode of operation is straightforward. In general, the combination of MALDI and SIMS is shown to add capabilities to each technique, providing a robust platform for TOF-SIMS experiments that already exists in a large number of laboratories.
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影响因子：
1.3
作者：
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通讯作者：
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