Modulating DNA translocation by a controlled deformation of a PDMS nanochannel device.
Modulating DNA translocation by a controlled deformation of a PDMS nanochannel device.
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DOI：
10.1038/srep00791
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发表时间：
2012
影响因子：
4.6
通讯作者：
Valbusa, Ugo
中科院分区：
文献类型：
--
作者：
Fanzio, Paola;Manneschi, Chiara;Angeli, Elena;Mussi, Valentina;Firpo, Giuseppe;Ceseracciu, Luca;Repetto, Luca;Valbusa, Ugo
已经开发了几种策略来控制纳米孔和纳米通道中的DNA易位。然而，对于超快速测序等单分子分析领域的应用来说，降低分子速度的可能性仍然具有挑战性。本文展示了通过动态改变其横截面的弹性纳米通道装置来改变DNA易位过程的可能性。更详细地，纳米通道变形由聚合物装置的宏观机械压缩引起。这种纳米通道的挤压可以减缓DNA分子在其中通过的速度。这种简单而低成本的方法是基于对装置的弹性性质的开发，可以与不同的传感技术相结合，适用于许多研究领域，例如DNA检测和操作，并且有希望在测序技术中进一步发展。
Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation, and is promising for further development in sequencing technology.
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