A single genomic region involving a putative chromosome rearrangement in flat oyster (Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae.
A single genomic region involving a putative chromosome rearrangement in flat oyster (Ostrea edulis) is associated with differential host resilience to the parasite Bonamia ostreae.
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DOI：
10.1111/eva.13446
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发表时间：
2022-09
影响因子：
4.1
通讯作者：
中科院分区：
文献类型：
--
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欧洲平贝(Ostrea Edulis)是一种具有重要生态和经济价值的海洋双壳贝类，受到胞内寄生虫的严重影响。在这项研究中，使用扁平牡蛎SNP阵列(~14,000个SNP)来验证先前报道的与东北大西洋地区赤潮杆菌暴露相关的发散选择的离群值基因座。对从北海收集的134只野生和孵化的个体进行了分析，这些个体来自幼稚(NV)和长期感染(LTA)地区。当使用中性标记时，遗传多样性和分化与采样来源(野生和孵化)有关，而当使用离群基因座进行差异选择时，遗传多样性和分化与良种状态(NV和LTA)有关。当使用异常值基因座时，两个基因簇在所有采样位置混合在一起，它们的频率与它们的良种状态有关。当比较两组时，异常值数据集显示出高度的遗传分化(F ST >； 0.25)，而不同于中性基因座(F ST不是≠ 0)。此外，与LTA样本相关的聚类显示出更高的遗传多样性和显著的杂合子过剩与异常基因座，但不具有中性数据。大多数离群值定位在扁平牡蛎基因组的8号染色体(OE-C8)上，支持一个主要的基因组区域，支持对博纳米氏病的复原力。此外，先前报道的NV株和LTA株之间的差异表达基因在OE-C8上显示出更高的定位密度。在OE-C8上注释的基因中，一系列相关的免疫功能被特异性地丰富，为细胞内寄生虫的弹性机制提供了假说。结果表明，分子标记辅助选择可用于培育对褐飞虱有抵抗力的品系，前提是感染褐飞虱后的LTA基因簇具有较低的寄生量和/或较高的生存能力。
European flat oyster (Ostrea edulis) is an ecologically and economically important marine bivalve, that has been severely affected by the intracellular parasite Bonamia ostreae. In this study, a flat oyster SNP array (~14,000 SNPs) was used to validate previously reported outlier loci for divergent selection associated with B. ostreae exposure in the Northeast Atlantic Area. A total of 134 wild and hatchery individuals from the North Sea, collected in naïve (NV) and long‐term affected (LTA) areas, were analysed. Genetic diversity and differentiation were related to the sampling origin (wild vs. hatchery) when using neutral markers, and to bonamiosis status (NV vs. LTA) when using outlier loci for divergent selection. Two genetic clusters appeared intermingled in all sampling locations when using outlier loci, and their frequency was associated with their bonamiosis status. When both clusters were compared, outlier data sets showed high genetic divergence (F ST > 0.25) unlike neutral loci (F ST not ≠ 0). Moreover, the cluster associated with LTA samples showed much higher genetic diversity and significant heterozygote excess with outlier loci, but not with neutral data. Most outliers mapped on chromosome 8 (OE‐C8) of the flat oyster genome, supporting a main genomic region underlying resilience to bonamiosis. Furthermore, differentially expressed genes previously reported between NV and LTA strains showed higher mapping density on OE‐C8. A range of relevant immune functions were specifically enriched among genes annotated on OE‐C8, providing hypotheses for resilience mechanisms to an intracellular parasite. The results suggest that marker‐assisted selection could be applied to breed resilient strains of O. edulis to bonamiosis, if lower parasite load and/or higher viability of the LTA genetic cluster following B. ostreae infection is demonstrated.
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影响因子：
3.7
作者：
Besnier F;Glover KA
通讯作者：
Glover KA
DOI：
10.1534/g3.117.041780
发表时间：
2017-07-05
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Gutierrez AP;Turner F;Gharbi K;Talbot R;Lowe NR;Peñaloza C;McCullough M;Prodöhl PA;Bean TP;Houston RD
通讯作者：
Houston RD
影响因子：
2.5
作者：
Engelsma, Marc Y.;Kerkhoff, Sonja;Hummel, Herman
通讯作者：
Hummel, Herman
影响因子：
2.7
作者：
Chambers, Lisa G.;Gaspar, Stephanie A.;Walters, Linda J.
通讯作者：
Walters, Linda J.
影响因子：
3.4
作者：
Holbrook, Zoe;Bean, Tim P.;Hauton, Chris
通讯作者：
Hauton, Chris