Recombinant Turkey Herpesvirus Expressing H9N2 HA Gene at the HVT005/006 Site Induces Better Protection Than That at the HVT029/031 Site.
Recombinant Turkey Herpesvirus Expressing H9N2 HA Gene at the HVT005/006 Site Induces Better Protection Than That at the HVT029/031 Site.
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DOI：
10.3390/v14112495
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发表时间：
2022-11-11
期刊：
影响因子：
--
通讯作者：
Qin A
中科院分区：
文献类型：
--
作者：
Zai X;Shi B;Shao H;Qian K;Ye J;Yao Y;Nair V;Qin A
土耳其疱疹病毒（HVT）作为一种有效的重组疫苗载体，被广泛用于表达来自HVT基因组不同位点的多种禽类病原体的保护性抗原。其中包括用于插入IBDV VP2的HVT029/031 （UL22-23）位点，以及最近发现的用于表达异源蛋白的新位点HVT005/006。为了比较不同部位HA基因重组疫苗的效果，我们首先在体外检测了HVT-005/006-hCMV-HA、HVT-005/006-MLV-HA和HVT-029/031-MLV-HA的生长曲线和HA表达水平。虽然3个重组病毒的生长动力学与亲本HVT没有显著差异，但HVT005/006位点的HA基因表达量高于HVT029/031位点。用1日龄SPF鸡对3种重组病毒的抗H9N2禽流感病毒效果进行了评价。与接种HVT-029/031-MLV-HA的鸡相比，接种HVT-005/006-MLV-HA或HVT-005/006-hCMV-HA的鸡的病毒脱落减少。此外，hvt -005/006- ha接种鸡的整体血凝抑制（HI）抗体滴度高于hvt -029/031- ha接种鸡。然而，HVT-005/006-MLV-HA和HVT-005/006-hCMV-HA在体外没有导致HA表达水平的显著差异，并且在攻毒后5天提供相同的保护效果（100%）。本研究结果表明，重组HVT005/006疫苗比重组HVT029/031疫苗能更好地表达HA， HVT-005/006-MLV-HA或HVT-005/006-hCMV-HA可能是保护鸡抗H9N2流感的候选疫苗。
Turkey herpesvirus (HVT) is widely used as an effective recombinant vaccine vector for expressing protective antigens of multiple avian pathogens from different loci of the HVT genome. These include the HVT029/031 (UL22–23) locus for the insertion of IBDV VP2 and the recently identified HVT005/006 locus as a novel site for expressing heterologous proteins. In order to compare the efficacy of recombinant vaccines with the HA gene at different sites, the growth curves and the HA expression levels of HVT-005/006-hCMV-HA, HVT-005/006-MLV-HA, and HVT-029/031-MLV-HA were first examined in vitro. While the growth kinetics of three recombinant viruses were not significantly different from those of parent HVT, higher expression of the HA gene was achieved from the HVT005/006 site than that from the HVT029/031 site. The efficacy of the three recombinant viruses against avian influenza H9N2 virus was also evaluated using one-day-old SPF chickens. Chickens immunized with HVT-005/006-MLV-HA or HVT-005/006-hCMV-HA displayed reduced virus shedding compared to HVT-029/031-MLV-HA vaccinated chickens. Moreover, the overall hemagglutination inhibition (HI) antibody titers of HVT-005/006-HA-vaccinated chickens were higher than that of HVT-029/031-HA-vaccinated chickens. However, HVT-005/006-MLV-HA and HVT-005/006-hCMV-HA did not result in a significant difference in the level of HA expression in vitro and provided the same protective efficacy (100%) at 5 days after challenge. In the current study, the results suggested that recombinant HVT005/006 vaccines caused better expression of HA than recombinant HVT029/031 vaccine, and that HVT-005/006-MLV-HA or HVT-005/006-hCMV-HA could be a candidate vaccine for the protection of chickens against H9N2 influenza.
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