The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1.
The Isolation and Characterization of a Broad Host Range Bcep22-like Podovirus JC1.
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DOI：
10.3390/v14050938
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发表时间：
2022-04-29
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噬菌体JC 1是一种短尾病毒科噬菌体，具有C1形态型，分离于宿主菌株新洋葱伯克霍尔德氏菌Van 1上。噬菌体JC 1能够感染广泛范围的洋葱伯克霍尔德菌复合体（Bcc）物种。JC 1基因组与Bcep 22样芽孢杆菌和许多罗尔斯通氏菌具有显著的相似性和同线性。JC 1基因组全长61,182 bp，G + C含量为65.4%，编码76个蛋白质和1个tRNA基因。与其他Lessieviruses不同的是，JC 1在其复制模块中编码一个推定的解旋酶基因，并且它处于一个独特的组织中，在以前分析的病毒中没有发现。JC 1基因组还含有3个有趣的低能基因，编码碳储存调节因子（CsrA），N-乙酰转移酶和磷酸腺苷磷酸硫酸（PAPS）还原酶。JC 1能稳定溶原化其宿主Van 1并整合到rimO基因的5′端。这是第一个帐户的稳定整合确定的Bcep 22样的p2p。JC 1在37 °C下的总体毒力指数高于30 °C下的总体毒力指数（分别为0.8和0.21）;然而，感染效率和溶原稳定性不受温度变化的影响，并且似乎不存在溶解性和溶原性生活方式之间可观察到的温度敏感性转换。尽管JC 1可以稳定地溶原化其宿主，但它具有用于噬菌体治疗的一些期望的特性。噬菌体JC 1具有广泛的宿主范围，并且需要细菌LPS的内核进行感染。突变以逃避JC 1感染的细菌可能会产生适应性劣势，如在先前表征的缺乏内核的LPS突变体中所见。
Bacteriophage JC1 is a Podoviridae phage with a C1 morphotype, isolated on host strain Burkholderia cenocepacia Van1. Phage JC1 is capable of infecting an expansive range of Burkholderia cepacia complex (Bcc) species. The JC1 genome exhibits significant similarity and synteny to Bcep22-like phages and to many Ralstonia phages. The genome of JC1 was determined to be 61,182 bp in length with a 65.4% G + C content and is predicted to encode 76 proteins and 1 tRNA gene. Unlike the other Lessieviruses, JC1 encodes a putative helicase gene in its replication module, and it is in a unique organization not found in previously analyzed phages. The JC1 genome also harbours 3 interesting moron genes, that encode a carbon storage regulator (CsrA), an N-acetyltransferase, and a phosphoadenosine phosphosulfate (PAPS) reductase. JC1 can stably lysogenize its host Van1 and integrates into the 5′ end of the gene rimO. This is the first account of stable integration identified for Bcep22-like phages. JC1 has a higher global virulence index at 37 °C than at 30 °C (0.8 and 0.21, respectively); however, infection efficiency and lysogen stability are not affected by a change in temperature, and no observable temperature-sensitive switch between lytic and lysogenic lifestyle appears to exist. Although JC1 can stably lysogenize its host, it possesses some desirable characteristics for use in phage therapy. Phage JC1 has a broad host range and requires the inner core of the bacterial LPS for infection. Bacteria that mutate to evade infection by JC1 may develop a fitness disadvantage as seen in previously characterized LPS mutants lacking inner core.
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3.2
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