Chemonucleolysis With Human Stromelysin‐1
Chemonucleolysis With Human Stromelysin‐1
复制标题
使用人 Stromelysin-1 进行化学溶核
作者：
H. Haro;S. Murakami;H. Komori;A. Okawa;K. Shinomiya
研究设计.对手术取出的突出髓核样本进行免疫组织学分析，以检查基质溶解素-1的表达。我们进行了体外和体内实验，以确定重组人（rh）基质溶解素-1是否能够降解髓核。Objective.分析基质溶解素-1在各种类型突出的髓核中的产生，并检查该重组蛋白对髓核组织的影响。背景数据总结。作者先前使用磁共振成像证实了一些经韧带和隔离型突出髓核的突出髓核大小逐渐减小。最近有报道称，在突出的髓核中，基质溶解素-1（一种软骨蛋白聚糖降解酶）的产生增加。作者推测，如果基质溶解素-1参与了突出的髓核的降解，则基质溶解素-1本身可用作化学溶核剂。方法.使用链霉亲和素-生物素方法对20个突出的髓核样本进行免疫组织学分析，以研究基质溶解素-1的表达。在存在或不存在rh基质溶解素-1的情况下，在组织培养基中孵育5个突出的髓核样本。孵育24小时后，测量其重量变化，并通过番红O染色评估蛋白聚糖的损失。取大鼠尾椎椎间盘髓核组织，进行自体皮下移植。将Rh基质溶解素-1注射到移植材料中，然后使用工程卡尺从皮肤表面进行二维测量。结果免疫组织学分析表明，基质分解素-1在突出的髓核的肉芽组织中产生。当将基质溶解素-1注射到小鼠髓核组织中时，它们的尺寸比对照组更快地减小。此外，在器官培养系统中用基质溶解素-1处理时，手术中获得的人类突出髓核材料显示出显著的重量减轻。番红O染色显示在这些突出的髓核样本中蛋白多糖的广泛消耗。结论.基质溶解素-1可能是突出的髓核吸收的关键酶，基质溶解素-1可能是用于化学髓核溶解的良好候选物。给予人基质溶解素-1可在生理上促进突出的髓核的吸收过程，提高愈合率，并减少化学髓核溶解术后的并发症。
Study Design. Immunohistologic analysis was performed on surgically removed samples of herniated nucleus pulposus to examine the expression of stromelysin‐1. We performed in vitro and in vivo experiments to determine whether recombinant human (rh) stromelysin‐1 is capable of degrading nucleus pulposus. Objective. To analyze the production of stromelysin‐1 in various types of herniated nucleus pulposus, and to examine the effects of this recombinant protein on nucleus pulposus tissues. Summary of Background Data. The authors previously demonstrated a progressive decrease in herniated nucleus pulposus size in some of the transligamentous and sequestration types of herniated nucleus pulposus using magnetic resonance imaging. An increased production of stromelysin‐1, a cartilage proteoglycan degrading enzyme, in herniated nucleus pulposus was reported recently. The authors speculated that if stromelysin‐1 is involved in the degradation of herniated nucleus pulposus, stromelysin‐1 itself may be used as a chemonucleolytic agent. Methods. Immunohistologic analysis using streptoavidin‐biotin method was performed on 20 herniated nucleus pulposus samples to investigate the expression of stromelysin‐1. Five herniated nucleus pulposus samples were incubated in a tissue culture medium in the presence or absence of rh stromelysin‐1. After 24 hours of incubation, their weight changes were measured, and the loss of proteoglycan was assessed by Safranin O staining. Rat nucleus pulposus tissues were obtained from coccygeal intervertebral discs, and autologous subcutaneous transplantation was performed. Rh stromelysin‐1 was injected into the grafted materials, and the reduction in size was followed by two‐dimensional measurements from the skin surface, using engineer's calipers. Results. Immunohistologic analysis demonstrated the production of stromelysin‐1 in the granulation tissues of herniated nucleus pulposus. When stromelysin‐1 was injected into the murine nucleus pulposus tissues, they reduced in size more rapidly than the control group. In addition, human herniated nucleus pulposus materials obtained at surgery showed significant weight loss when treated with stromelysin‐1 in an organ culture system. Safranin O staining revealed extensive depletion of proteoglycan in these herniated nucleus pulposus samples. Conclusions. Stromelysin‐1 is a possible key enzyme in herniated nucleus pulposus resorption, and stromelysin‐1 may be a good candidate for use in chemonucleolysis. Administration of human stromelysin‐1 may physiologically facilitate the resorption process of herniated nucleus pulposus, increase the healing rate, and decrease complications after chemonucleolysis.
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DOI：
10.2106/00004623-198365090-00002
发表时间：
1983-12
期刊：
The Journal of bone and joint surgery. American volume
影响因子：
--
作者：
D. Bradford;K. M. Cooper;T. Oegema
通讯作者：
D. Bradford;K. M. Cooper;T. Oegema
影响因子：
27.4
作者：
OKADA, Y;TAKEUCHI, N;NAGASE, H
通讯作者：
NAGASE, H
影响因子：
15.9
作者：
DEAN, DD;MARTELPELLETIER, J;WOESSNER, JF
通讯作者：
WOESSNER, JF
影响因子：
15.9
作者：
RODGERS, WH;MATRISIAN, LM;OSTEEN, KG
通讯作者：
OSTEEN, KG
影响因子：
--
作者：
BONASSAR, LJ;FRANK, EH;GRODZINSKY, AJ
通讯作者：
GRODZINSKY, AJ