Microbiologic profile of endodontic infections from HIV- and HIV+ patients using multiple-displacement amplification and checkerboard DNA-DNA hybridization.
Microbiologic profile of endodontic infections from HIV- and HIV+ patients using multiple-displacement amplification and checkerboard DNA-DNA hybridization.
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DOI：
10.1111/j.1601-0825.2012.01908.x
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发表时间：
2012-09
期刊：
影响因子：
3.8
通讯作者：
Teles FR
中科院分区：
文献类型：
--
作者：
Brito LC;Sobrinho AP;Teles RP;Socransky SS;Haffajee AD;Vieira LQ;Teles FR
口腔疾病（2012）18,558–567目的：比较 HIV 阴性和 HIV 阳性受试者坏死牙髓中牙髓感染的微生物群。材料和方法：从 40 名 HIV− 和 20 名 HIV+ 受试者中采集坏死牙髓的根管样本。使用多重置换扩增（MDA）对纸浆进行扩增。然后，采用棋盘 DNA-DNA 杂交来评估 107 个微生物类群的水平。研究了 DNA 探针计数的百分比和每个测试物种定植的牙齿的百分比。分别使用曼-惠特尼检验和卡方分析来寻找组间在分类群比例和测试物种患病率方面的显着差异。结果：在 HIV 受试者和 D. 受试者中，检测到的最常见分类群是肺炎杆菌、嗜麦芽寡养单胞菌、远缘链球菌、白喉杆菌和幽门螺杆菌。 HIV + 个体中的肺炎链球菌、坦纳普氏菌、牙龈卟啉单胞菌、微单胞菌、黑普氏菌和白喉棒状杆菌。肺动脉炎，C.白喉，和C. abicanswere 是 HIV- 组中最丰富的物种，而 HIV+ 样本中的主要分类单元是 P。坦纳拉，D.肺炎和奥尔森氏菌坦纳拉，O. HIV+样本中uli、Veilonella dispar、Bacteroides fragilis和Actinomyces meyeri的含量明显更高。结论：HIV-和HIV+个体之间特定微生物类群的流行率和比例存在显着差异。根管微生物群可能是重要的口腔和医学病原体的储存库，主要存在于艾滋病毒+个体中。
Oral Diseases(2012)18,558–567Objective:To compare the microbiota of endodontic infections in necrotic pulp from HIV‐negative and HIV‐positive subjects.Materials and Methods:Root canal samples from necrotic pulp were collected from 40 HIV− and 20 HIV+ subjects. Pulps were amplified using multiple displacement amplification (MDA). Then, checkerboard DNA–DNA hybridization was employed to assess the levels of 107 microbial taxa. The percentage of DNA probe count and the percentage of teeth colonized by each test species were investigated. Significant differences between groups regarding proportions of taxa and prevalence of the test species were sought using the Mann–Whitney test and the Chi‐square analysis, respectively.Results:The most prevalent taxa detected wereDialister pneumosintes,Stenotrophomonas maltophilia,Streptococcus sobrinus,Corynebacterium diphteriae, andHelicobacter pyloriamong HIV‐ subjects andD. pneumosintes,Prevotella tannerae,Porphyromonas gingivalis,Parvimonas micra,Prevotella nigrescens, andCorynebacterium diphtheriaeamong HIV+ individuals.D. pneumosintes,C. diphtheria, andC. albicanswere the most abundant species in the HIV‐ group, whereas the predominant taxa in HIV+ samples wereP. tannerae,D. pneumosintesandOlsenella uli.P. tannerae,O. uli,Veilonella dispar,Bacteroides fragilis, andActinomyces meyeriwere significantly more abundant in HIV+ samples.Conclusions:There were significant differences in the prevalence and proportions of specific microbial taxa between HIV− and HIV+ individuals. The root canal microbiota may represent a reservoir of important oral and medical pathogens, mainly in HIV+ individuals.
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1007/s10096-009-0786-5
发表时间：
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