Voltage-sensitive dye imaging reveals improved topographic activation of cortex in response to manipulation of thalamic microstimulation parameters.
Voltage-sensitive dye imaging reveals improved topographic activation of cortex in response to manipulation of thalamic microstimulation parameters.
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DOI：
10.1088/1741-2560/9/2/026008
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发表时间：
2012-04
影响因子：
4
通讯作者：
Stanley GB
中科院分区：
文献类型：
--
作者：
Wang Q;Millard DC;Zheng HJ;Stanley GB
电压敏感染料（VSD）成像被用来量化在体内，网络水平的时空皮层激活反应的丘脑在大鼠触须通路的电微刺激。丘脑微刺激诱发了一个明显不同的皮质反应比自然的感觉刺激，与微刺激的反应蔓延到一个更大的面积的皮质和地形失调的皮质柱刺激丘脑区域项目。电刺激与阴极领先的不对称波形减少了这种地形失调，同时增加了空间特异性的皮层激活。系统地增加微刺激脉冲的不对称性揭示了对称和不对称刺激之间的连续性，逐渐降低了地形偏差。这些数据有力地支持了这样的假设，即电刺激波形的操纵可以用于选择性地激活特定的神经元件。具体来说，我们的研究结果是一致的预测，阴极主导的非对称波形优先刺激轴突的细胞体，而对称波形优先激活轴突的细胞体。这里的研究结果提供了一些初步的步骤，设计和优化神经电路的微刺激，并打开了大门，以更复杂的工程工具，如非线性系统识别技术，开发技术，更有效地控制神经系统的活动。
Voltage sensitive dye (VSD) imaging was used to quantify in-vivo, network level spatiotemporal cortical activation in response to electrical microstimulation of the thalamus in the rat vibrissa pathway. Thalamic microstimulation evoked a distinctly different cortical response than natural sensory stimulation, with the response to microstimulation spreading over a larger area of cortex and being topographically misaligned with the cortical column to which the stimulated thalamic region projects. Electrical stimulation with cathode-leading asymmetric waveforms reduced this topographic misalignment while simultaneously increasing the spatial specificity of the cortical activation. Systematically increasing the asymmetry of the microstimulation pulses revealed a continuum between symmetric and asymmetric stimulation that gradually reduced the topographic bias. These data strongly support the hypothesis that manipulation of the electrical stimulation waveform can be used to selectively activate specific neural elements. Specifically, our results are consistent with the prediction that cathode-leading asymmetric waveforms preferentially stimulating cell bodies over axons, while symmetric waveforms preferentially activate axons over cell bodies. The findings here provide some initial steps toward the design and optimization of microstimulation of neural circuitry, and open the door to more sophisticated engineering tools, such as nonlinear system identification techniques, to develop technologies for more effective control of activity in the nervous system.
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影响因子：
1.8
作者：
Humayun, MS;Weiland, JD;de Juan, E
通讯作者：
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