Neuropeptidomic Profiling and Localization in the Crustacean Cardiac Ganglion Using Mass Spectrometry Imaging with Multiple Platforms.
Neuropeptidomic Profiling and Localization in the Crustacean Cardiac Ganglion Using Mass Spectrometry Imaging with Multiple Platforms.
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DOI：
10.1021/jasms.0c00191
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发表时间：
2020-12-02
影响因子：
3.2
通讯作者：
Li L
中科院分区：
文献类型：
--
作者：
DeLaney K;Li L
甲壳动物的心脏神经肌肉系统是研究神经回路如何产生行为的有用模型，因为它由一个包含九个神经元的简单神经节组成，但作为一个强大的中枢模式生成器。甲壳类心脏是神经源性的，接受神经肽的输入。然而，神经肽对心输出量的具体作用尚不完全清楚，而且多个神经肽之间的高度共调使得研究这些影响更具挑战性。为了解决这一挑战，使用基质辅助激光解吸/电离(MALDI)质谱仪(MS)成像来定位心脏神经节(CG)内的神经肽，提供有关神经肽的身份和定位的信息。CG提取物也通过数据独立采集(DIA)方法与串联质谱仪(MS/MS)联用，得到了316个神经肽的确证。为了提供全面的结果，比较了两个MS成像(MSI)平台，包括MALDI-Orbitrap仪器和MALDI-TOF/TOF，前者用于高质量谱分辨率进行准确识别，后者用于提高空间分辨率和灵敏度，提供更具描述性的MS图像。获得了235个推测的神经肽的MS图像，其中145个被补充的MS/MS数据或精确的质量匹配所确认。MSI研究展示了基于MALDI的原位分析策略在揭开小型9细胞神经系统中存在的化学复杂性方面的敏感性和能力。这项研究的结果将使对这一重要神经回路中神经肽功能的更多信息分析成为可能。
The crustacean cardiac neuromuscular system is a useful model for studying how neural circuits generate behavior, as it is comprised of a simple ganglion containing nine neurons, yet acts as a robust central pattern generator. The crustacean heart is neurogenic, receiving input from neuropeptides. However, the specific effects of neuropeptides on cardiac output is not fully understood, and the large degree of comodulation between multiple neuropeptides makes studying these effects more challenging. To address this challenge, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging was used to localize neuropeptides within the cardiac ganglion (CG), providing information about the identity and localization of neuropeptides being present. CG extracts were also profiled using liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) with a data independent acquisition (DIA) method, resulting in the confirmation of 316 neuropeptides. Two MS imaging (MSI) platforms were compared in order to provide comprehensive results, including a MALDI-Orbitrap instrument for high mass spectral resolution for accurate identifications and a MALDI-TOF/TOF for improved spatial resolution and sensitivity, providing more descriptive MS images. MS images for 235 putative neuropeptides were obtained, with the identification of 145 of these being confirmed by either complementary MS/MS data or accurate mass matching. The MSI studies demonstrate the sensitivity and power of MALDI-based in situ analytical strategy for unraveling the chemical complexity present in a small 9-cell neuronal system. The results of this study will enable more informative assays of the functions of neuropeptides within this important neural circuit.
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影响因子：
2.7
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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