Pyocyanin facilitates extracellular DNA binding to Pseudomonas aeruginosa influencing cell surface properties and aggregation.
Pyocyanin facilitates extracellular DNA binding to Pseudomonas aeruginosa influencing cell surface properties and aggregation.
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DOI：
10.1371/journal.pone.0058299
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Manefield M
中科院分区：
文献类型：
--
作者：
Das T;Kutty SK;Kumar N;Manefield M
绿脓菌素是由人类病原体铜绿假单胞菌产生的电化学活性代谢产物。它是一种公认的毒力因子，参与多种重要的生物学活性，包括基因表达，维持细菌细胞的适应性和生物膜形成。它也被认为是细菌呼吸的电子穿梭，以及抗菌和抗真菌剂。eDNA也已被证明是建立铜绿假单胞菌生物膜的主要组分。在这项研究中，我们发现绿脓菌素的产生影响eDNA与铜绿假单胞菌PA 14细胞的结合，介导的绿脓菌素与eDNA的嵌入。铜绿假单胞菌的细胞表面性质，包括细胞大小（流体动力学直径），疏水性和有吸引力的表面能的影响由eDNA在绿脓菌素的存在下，影响物理化学相互作用和促进聚集。与野生型菌株相比，ΔphzA-G PA 14突变体不能与eDNA结合，导致流体动力学直径减小，疏水性降低，排斥性物理化学相互作用和聚集减少。通过DNA酶I处理PA 14野生型菌株去除eDNA导致聚集、细胞表面疏水性和大小显著降低，并且排斥性物理化学相互作用增加，与ΔphzA-G突变体的水平相似。ΔphzA-G突变体的细胞表面性质不受DNase I处理的影响。基于这些发现，我们提出绿脓菌素插入eDNA通过影响铜绿假单胞菌细胞表面性质和物理化学相互作用来促进铜绿假单胞菌细胞中的细胞与细胞相互作用。
Pyocyanin is an electrochemically active metabolite produced by the human pathogen Pseudomonas aeruginosa. It is a recognized virulence factor and is involved in a variety of significant biological activities including gene expression, maintaining fitness of bacterial cells and biofilm formation. It is also recognized as an electron shuttle for bacterial respiration and as an antibacterial and antifungal agent. eDNA has also been demonstrated to be a major component in establishing P. aeruginosa biofilms. In this study we discovered that production of pyocyanin influences the binding of eDNA to P. aeruginosa PA14 cells, mediated through intercalation of pyocyanin with eDNA. P. aeruginosa cell surface properties including cell size (hydrodynamic diameter), hydrophobicity and attractive surface energies were influenced by eDNA in the presence of pyocyanin, affecting physico-chemical interactions and promoting aggregation. A ΔphzA-G PA14 mutant, deficient in pyocynain production, could not bind with eDNA resulting in a reduction in hydrodynamic diameter, a decrease in hydrophobicity, repulsive physico-chemical interactions and reduction in aggregation in comparison to the wildtype strain. Removal of eDNA by DNase I treatment on the PA14 wildtype strain resulted in significant reduction in aggregation, cell surface hydrophobicity and size and an increase in repulsive physico-chemical interactions, similar to the level of the ΔphzA-G mutant. The cell surface properties of the ΔphzA-G mutant were not affected by DNase I treatment. Based on these findings we propose that pyocyanin intercalation with eDNA promotes cell-to-cell interactions in P. aeruginosa cells by influencing their cell surface properties and physico-chemical interactions.
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影响因子：
3.2
作者：
Petersen, FC;Tao, L;Scheie, AA
通讯作者：
Scheie, AA
影响因子：
3.6
作者：
Allesen-Holm, M;Barken, KB;Tolker-Nielsen, T
通讯作者：
Tolker-Nielsen, T
影响因子：
2.6
作者：
Liu, Hui-Hui;Yang, Yi-Ran;Xie, Zhi-Xiong
通讯作者：
Xie, Zhi-Xiong
影响因子：
2.8
作者：
Boks, Niels P.;Norde, Willem;Busscher, Henk J.
通讯作者：
Busscher, Henk J.
影响因子：
3.5
作者：
SUH, D;CHAIRES, JB
通讯作者：
CHAIRES, JB