Mitigation of Articular Cartilage Degeneration and Subchondral Bone Sclerosis in Osteoarthritis Progression Using Low-Intensity Ultrasound Stimulation.
Mitigation of Articular Cartilage Degeneration and Subchondral Bone Sclerosis in Osteoarthritis Progression Using Low-Intensity Ultrasound Stimulation.
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DOI：
10.1016/j.ultrasmedbio.2018.08.022
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发表时间：
2019-01
影响因子：
2.9
通讯作者：
Qin YX
中科院分区：
文献类型：
--
作者：
Li X;Sun Y;Zhou Z;Zhang D;Jiao J;Hu M;Hassan CR;Qin YX
本研究的目的是评价低强度超声波在正常和功能性废弃状态下对骨关节炎进展过程中关节软骨和软骨下骨的影响。将60只5月龄雌性SD大鼠随机分为6组(n=10/组)：年龄匹配组、OA组、OA+超声(US)组、后肢悬吊(HLS)组、HLS+OA组、HLS+OA+US组。取手术切除的前交叉韧带制作右膝关节骨性关节炎模型。诱导2周后，用3 MHz换能器，脉冲占空比为20%，声强为30 mW/cm2，对右膝关节进行低强度超声治疗，每次20min，每周5天，共6周。取右侧胫骨进行Micro-CT、组织学和力学分析。Micro-CT结果显示，在正常关节使用条件下，OA组软骨厚度和硫酸糖胺聚糖(SGAG)含量降低，而软骨下皮质骨板厚度和软骨下松质骨形成增加。此外，组织学结果显示，在骨性关节炎进展过程中，软骨细胞密度和排列受损，软骨下骨增加。这些变化伴随着骨性关节炎软骨力学参数的降低。而在联合废用状态下，HLS+OA组的骨性关节炎症状较少。美国治疗组软骨退变和软骨下骨硬化减轻，尤其是在正常关节使用条件下。综上所述，低强度超声可改善骨关节炎进展过程中的软骨退变和软骨下硬化。同时，也为今后骨性关节炎患者的临床治疗提供了一种很有前途的策略。
The purpose of this study was to evaluate the effect of low-intensity ultrasound on articular cartilage and subchondral bone alterations in joints under normal and functional disuse conditions during osteoarthritis (OA) progression. Sixty 5-month-old female Sprague-Dawley rats were randomly assigned to six groups (n=10/group): Age-match group, OA group, OA+ultrasound (US) group, Hindlimbs suspension (HLS) group, HLS+OA group, and HLS+OA+US group. The surgical anterior cruciate ligament was used to induce OA in the right knee joints. After two weeks of OA induction, low-intensity ultrasound generated by a 3 MHz transducer with 20% pulse duty cycle and 30 mW/cm2 acoustic intensity was conducted in right knee joints for 20 mins a day, five days a week, and a total of 6 weeks. Then, the right tibias were harvested for Micro-CT, histological and mechanical analysis. Micro-CT results indicated that the thickness and sulfated glycosaminoglycan (sGAG) content of cartilage decreased, but the thickness of the subchondral cortical bone plate and the formation of subchondral trabecular bone increased in the OA group under the normal joint use condition. Furthermore, histological results demonstrated that chondrocyte density and arrangement in cartilage corrupted and the underlying subchondral bone increased during OA progression. These changes were accompanied by reductions of mechanical parameters in OA cartilages. However, less OA symptoms were observed in HLS+OA group under the joint disuse condition. The cartilage degeneration and subchondral bone sclerosis were alleviated in the US treatment group, especially under normal joint use condition. In conclusion, low-intensity ultrasound could improve cartilage degeneration and subchondral sclerosis during OA progression. Also, it could provide a promising strategy for future clinical treatment for OA patients.
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