Capsule influences the deposition of critical complement C3 levels required for the killing of Burkholderia pseudomallei via NADPH-oxidase induction by human neutrophils.
Capsule influences the deposition of critical complement C3 levels required for the killing of Burkholderia pseudomallei via NADPH-oxidase induction by human neutrophils.
复制标题
DOI：
10.1371/journal.pone.0052276
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Wooten RM
中科院分区：
文献类型：
--
作者：
Woodman ME;Worth RG;Wooten RM
类鼻疽伯克霍尔德菌是类鼻疽病的病原体，并且是其流行地区败血症的主要媒介。由于通过气溶胶的低LD50和对多种抗生素的耐药性，它被CDC和APHIS认为是一级选择剂。B。类鼻疽杆菌是一种包囊细菌，可感染、繁殖并在多种宿主细胞类型内持续存在。体内研究表明，巨噬细胞和中性粒细胞是重要的控制B。然而，关于中性粒细胞如何对这些细菌作出反应的细节知之甚少。我们的目标是描述人类中性粒细胞控制高毒力B的能力。与相对无毒、无囊的B相比，thailandensis使用体外分析。B。thailandensis比B更容易被吞噬。假鼻疽，但两者在中性粒细胞内表现出相似的持久性，表明它们具有相似的逃避中性粒细胞清除的固有能力。血清调理作用研究表明，两者都对补体的直接杀伤有抵抗力，尽管B。thailandensis在其表面获得的C3显著多于B。类鼻疽，其多糖胶囊显著降低了细菌表面上的补体沉积水平。这两种伯克霍尔德氏菌的物种表现出显着增强的摄取和杀伤中性粒细胞后，临界水平的C3沉积。血清调理伯克霍尔德氏菌诱导一个显着的呼吸爆发的中性粒细胞相比，unopsonized细菌，和中性粒细胞的杀伤被阻止抑制NADPH氧化酶。总之，中性粒细胞可以有效地杀死B。类鼻疽和B. Thailandensis具有补体沉积的临界阈值，并且它们抵抗表面调理作用的能力的相对差异可能有助于在体内观察到的不同毒力表型。
Burkholderia pseudomallei is the causative agent of melioidosis and is a major mediator of sepsis in its endemic areas. Because of the low LD50 via aerosols and resistance to multiple antibiotics, it is considered a Tier 1 select agent by the CDC and APHIS. B. pseudomallei is an encapsulated bacterium that can infect, multiply, and persist within a variety of host cell types. In vivo studies suggest that macrophages and neutrophils are important for controlling B. pseudomallei infections, however few details are known regarding how neutrophils respond to these bacteria. Our goal is to describe the capacity of human neutrophils to control highly virulent B. pseudomallei compared to the relatively avirulent, acapsular B. thailandensis using in vitro analyses. B. thailandensis was more readily phagocytosed than B. pseudomallei, but both displayed similar rates of persistence within neutrophils, indicating they possess similar inherent abilities to escape neutrophil clearance. Serum opsonization studies showed that both were resistant to direct killing by complement, although B. thailandensis acquired significantly more C3 on its surface than B. pseudomallei, whose polysaccharide capsule significantly decreased the levels of complement deposition on the bacterial surface. Both Burkholderia species showed significantly enhanced uptake and killing by neutrophils after critical levels of C3 were deposited. Serum-opsonized Burkholderia induced a significant respiratory burst by neutrophils compared to unopsonized bacteria, and neutrophil killing was prevented by inhibiting NADPH-oxidase. In summary, neutrophils can efficiently kill B. pseudomallei and B. thailandensis that possess a critical threshold of complement deposition, and the relative differences in their ability to resist surface opsonization may contribute to the distinct virulence phenotypes observed in vivo.
登录
查看更多内容
DOI：
10.1099/00207713-48-1-317
发表时间：
1998-01-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Brett, PJ;DeShazer, D;Woods, DE
通讯作者：
Woods, DE
影响因子：
3.1
作者：
Chanchamroen, Sujin;Kewcharoenwong, Chidchamai;Lertmemongkolchai, Ganjana
通讯作者：
Lertmemongkolchai, Ganjana
影响因子：
4.2
作者：
Brett, PJ;Deshazer, D;Woods, DE
通讯作者：
Woods, DE
影响因子：
2.7
作者：
Currie, BJ;Fisher, DA;Mayo, MJ
通讯作者：
Mayo, MJ
影响因子：
3.1
作者：
Conlan, JW
通讯作者：
Conlan, JW