Chemical and genomic characterization of a potential probiotic treatment for stony coral tissue loss disease.
Chemical and genomic characterization of a potential probiotic treatment for stony coral tissue loss disease.
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DOI：
10.1038/s42003-023-04590-y
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发表时间：
2023-04-06
影响因子：
5.9
通讯作者：
中科院分区：
文献类型：
--
作者：
石珊瑚组织丧失疾病(SCTLD)被认为是历史上最具破坏性的珊瑚疾病爆发之一，目前正在佛罗里达州的珊瑚礁和大加勒比海地区蔓延。SCTLD影响到至少24种不同的珊瑚物种，并与珊瑚覆盖的广泛损失有关。在这里，我们展示了假交替单胞菌。菌株McH1-7对SCTLD相关细菌具有广谱抗菌活性。化学分析表明McH1-7至少产生两种潜在的抗菌剂，高米星和四溴吡咯，而基因组分析确定了可能编码L氨基酸氧化酶和多种抗菌金属蛋白水解酶(假交替蛋白)的基因。在实验室试验中，mch1-7阻止或延缓了68.2%的海绵状藻病发片段(n = 22)的病情发展，并100%地阻止了疾病的传播(n = 12)。McH1-7是最具化学特征的珊瑚益生菌，是一种有效的预防和直接治疗破坏性SCTLD的方法，也是抗生素使用的潜在替代品。用多学科方法鉴定交替假单胞菌的广谱抗菌活性。菌株McH1-7对抗威胁加勒比海珊瑚礁的石珊瑚组织丧失疾病。
Considered one of the most devastating coral disease outbreaks in history, stony coral tissue loss disease (SCTLD) is currently spreading throughout Florida’s coral reefs and the greater Caribbean. SCTLD affects at least two dozen different coral species and has been implicated in extensive losses of coral cover. Here we show Pseudoalteromonas sp. strain McH1-7 has broad-spectrum antibacterial activity against SCTLD-associated bacterial isolates. Chemical analyses indicated McH1-7 produces at least two potential antibacterials, korormicin and tetrabromopyrrole, while genomic analysis identified the genes potentially encoding an L-amino acid oxidase and multiple antibacterial metalloproteases (pseudoalterins). During laboratory trials, McH1-7 arrested or slowed disease progression on 68.2% of diseased Montastraea cavernosa fragments treated (n = 22), and it prevented disease transmission by 100% (n = 12). McH1-7 is the most chemically characterized coral probiotic that is an effective prophylactic and direct treatment for the destructive SCTLD as well as a potential alternative to antibiotic use. A multidisciplinary approach identifies broad-spectrum antibacterial activity of Pseudoalteromonas sp. strain McH1-7 against stony coral tissue loss disease, which is threatening Caribbean coral reefs.
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影响因子：
3.7
作者：
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通讯作者：
Paull, Valerie J.
DOI：
10.1073/pnas.1519695113
发表时间：
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影响因子：
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影响因子：
15.5
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