Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus.
Gut microbial community supplementation and reduction modulates African armyworm susceptibility to a baculovirus.
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DOI：
10.1093/femsec/fiac147
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发表时间：
2022-12-14
影响因子：
4.2
通讯作者：
Wilson, Kenneth
中科院分区：
文献类型：
--
作者：
Donkersley, Philip;Rice, Annabel;Graham, Robert, I;Wilson, Kenneth
肠道微生物群刺激免疫系统并抑制病原体，因此，它对疾病预防至关重要。益生菌代表了用于治疗和预防细菌性疾病的抗生素的有效替代品。益生菌通常用于脊椎动物，尽管它们在无脊椎动物中的使用仍然很少。我们使用抗生素和野外收集的粪便操纵非洲粘虫（Spodoptera examputa步行者）的肠道微生物组，试图了解肠道微生物组与核型多角体病毒SpexNPV的相互作用。我们发现S.相对于具有典型实验室肠道微生物组的个体，具有补充肠道微生物组的个体对SpexNPV的抗性显著更高。Illumina MiSeq测序揭示了S.豁免肠属于28个不同的类。具有增加的乳酸杆菌目丰度的个体具有更高的病毒感染存活概率。相比之下，有一个增加的丰度肠杆菌和假单胞菌在死于病毒感染的个人，对应于这两个订单在幸存的毛毛虫的丰度下降，这表明它们在调节宿主和病原体之间的相互作用的潜在作用。这些结果对生物农药的实验室研究具有重要意义。我们的研究探讨了一种广泛分布的昆虫害虫物种（非洲粘虫）的实验室菌株如何具有独特的肠道微生物组，以及肠道微生物在影响对核型多角体病毒SpexNPV的抗性中所起的作用。通过抗生素控制和现场收集的粪便接种相结合，我们证明了将这种害虫物种的田间微生物组接种到实验室菌株中会增加对病毒的抗性，并且随后在用广谱抗生素处理时会逆转。
Gut microbiota stimulates the immune system and inhibits pathogens, and thus, it is critical for disease prevention. Probiotics represent an effective alternative to antibiotics used for the therapy and prevention of bacterial diseases. Probiotic bacteria are commonly used in vertebrates, although their use in invertebrates is still rare. We manipulated the gut microbiome of the African Armyworm (Spodoptera exempta Walker) using antibiotics and field-collected frass, in an attempt to understand the interactions of the gut microbiome with the nucleopolyhedrovirus, SpexNPV. We found that S. exempta individuals with supplemented gut microbiome were significantly more resistant to SpexNPV, relative to those with a typical laboratory gut microbiome. Illumina MiSeq sequencing revealed the bacterial phyla in the S. exempta gut belonged to 28 different classes. Individuals with an increased abundance of Lactobacillales had a higher probability of surviving viral infection. In contrast, there was an increased abundance of Enterobacteriales and Pseudomonadales in individuals dying from viral infection, corresponding with decreased abundance of these two Orders in surviving caterpillars, suggesting a potential role for them in modulating the interaction between the host and its pathogen. These results have important implications for laboratory studies testing biopesticides. Our study examines how lab strains of a widespread insect pest species (the African Armyworm) have a distinct gut microbiome and the role that gut microbes play in affecting resistance to nucleopolyhedrovirus, SpexNPV. Through a combination of antiobiotic control and field-collected frass innoculations, we demonstrate that inoculation of the field microbiome of this pest species into lab strains increases resistance to the virus, and that this is subsequently reversed when treated with a broad spectrum antibiotic.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
3.7
作者：
Fredensborg, Brian L.;Fossdal i Kalvalio, Inga;Kapel, Christian M. O.
通讯作者：
Kapel, Christian M. O.
影响因子：
6.7
作者：
Grzywacz, D.;Stevenson, P. C.;Wilson, K.
通讯作者：
Wilson, K.
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
3.2
作者：
Darriet, Frederic;Marcombe, Sebastien;Corbel, Vincent
通讯作者：
Corbel, Vincent