Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam.
Molecular identification of Fasciola spp. (Digenea: Platyhelminthes) in cattle from Vietnam.
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DOI：
10.1051/parasite/2012191085
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发表时间：
2012-02
期刊：
影响因子：
--
通讯作者：
Nakai Y
中科院分区：
文献类型：
--
作者：
Nguyen S;Amer S;Ichikawa M;Itagaki T;Fukuda Y;Nakai Y
片形吸虫属从越南庆和省当地屠宰场的自然感染牛中收集，用于形态学和遗传学研究。显微镜检查发现，在精囊中没有精子细胞，这表明孤雌生殖的吸虫。核糖体RNA第一和第二内转录间隔区（ITS1和ITS2）的序列分析表明，16个分离株中有13个为片形吸虫型，3个为片形吸虫杂合型。肝片吸虫和肝片吸虫。有趣的是，所有的线粒体序列（部分COI和NDI）都是F。表明该杂种的母系来源于F.南极洲未检测到序列内变异。
Fasciola spp. were collected from naturally infected cattle at a local abattoir of Khanh Hoa province, Vietnam, for morphological and genetic investigations. Microscopic examination detected no sperm cells in the seminal vesicles, suggesting a parthenogenetic reproduction of the flukes. Analyses of sequences from the first and second internal transcribed spacers (ITS1 and ITS2) of the ribosomal RNA revealed that 13 out of 16 isolates were of Fasciola gigantica type, whereas three isolates presented a hybrid sequence from F. gigantica and Fasciola hepatica. Interestingly, all the mitochondrial sequences (partial COI and NDI) were of F. gigantica type, suggesting that the maternal lineage of the hybrid form is from F. gigantica. No intra-sequence variation was detected.
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