Redox proteomics for the assessment of redox-related posttranslational regulation in plants.

Redox proteomics for the assessment of redox-related posttranslational regulation in plants.
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用于评估植物氧化还原相关翻译后调控的氧化还原蛋白质组学

DOI:
10.1016/j.bbapap.2016.01.005
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发表时间:
2016
期刊:
Biochimica et biophysica acta
影响因子:
--
通讯作者:
Dietz KJ
Dietz KJ
中科院分区:
--
文献类型:
--
作者:
Mock HP;Dietz KJ

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体内和体外蛋白质标记和后续检测的方法学发展使得氧化还原修饰的灵敏和特异性检测成为可能。这些方法目前应用于不同的细胞和组织,亚蛋白质组和发育以及环境条件。叶绿体蛋白质组是特别适合这样的研究,因为叶绿体蛋白质的氧化还原调节是建立良好的,许多质体蛋白质是丰富的,氧化还原网络组件已被库存在很大的深度,和功能的后果探索。因此,一方面,他们的丰富的另一方面,在不久的将来,了解他们的生理调节的贡献,氧化还原相关的翻译后修饰的剧目构成了挑战。介绍了各种翻译后氧化还原修饰,然后描述了可用的蛋白质组学方法。氧化还原相关的翻译后修饰的意义是示例性的工作,从光合作用建立的例子。这篇文章是由Hans-Peter Mock博士编辑的题为“植物蛋白质组学-基础过程和作物生产之间的桥梁”的特刊的一部分。
The methodological developments of in vivo and in vitro protein labeling and subsequent detection enable sensitive and specific detection of redox modifications. Such methods are presently applied to diverse cells and tissues, subproteomes and developmental as well as environmental conditions. The chloroplast proteome is particularly suitable for such kind of studies, because redox regulation of chloroplast proteins is well established, many plastid proteins are abundant, redox network components have been inventoried in great depth, and functional consequences explored. Thus the repertoire of redox-related posttranslational modifications on the one hand side and their abundance on the other pose a challenge for the near future to understand their contribution to physiological regulation. The various posttranslational redox modifications are introduced, followed by a description of the available proteomics methods. The significance of the redox-related posttranslational modification is exemplarily worked out using established examples from photosynthesis. This article is part of a Special Issue entitled: Plant Proteomics — a bridge between fundamental processes and crop production, edited by Dr. Hans-Peter Mock.
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