Gut Bacterial Species Distinctively Impact Host Purine Metabolites during Aging in Drosophila.
Gut Bacterial Species Distinctively Impact Host Purine Metabolites during Aging in Drosophila.
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DOI：
10.1016/j.isci.2020.101477
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发表时间：
2020-09-25
期刊：
影响因子：
5.8
通讯作者：
Obata F
中科院分区：
文献类型：
--
作者：
Yamauchi T;Oi A;Kosakamoto H;Akuzawa-Tokita Y;Murakami T;Mori H;Miura M;Obata F
肠道微生物群影响宿主代谢组并影响其健康寿命。肠道中的细菌种类如何影响年龄依赖性代谢改变尚未阐明。在这里，我们表明，在果蝇，尿囊素，嘌呤代谢的最终产物，在衰老过程中增加微生物群依赖的方式。尿囊素水平在幼蝇中很低，但在缩短寿命的饮食操作（如高嘌呤、高糖或高酵母喂养）后通常会升高。去除果蝇微生物组中的Acetobacter persici减弱了年龄依赖性尿囊素的增加。单协会与A. persici，但不与植物乳杆菌，增加尿囊素在老年苍蝇。波斯曲霉通过激活肾小管中的先天免疫信号转导IMD途径来增加尿囊素。另一方面，对细菌调节饮食的分析表明，植物乳杆菌可以通过减少饮食中的嘌呤来减少尿囊素。这些数据共同证明了肠道微生物组对宿主嘌呤水平的物种特异性调节。肠道细菌物种调节果蝇年龄依赖性代谢转变Acetobacter persici通过肾小管IMD激活增加嘌呤水平植物乳杆菌降低果蝇饮食中的嘌呤水平饮食和细菌依赖性嘌呤水平升高可能缩短寿命生物科学;分子生物学;免疫学;微生物组学
Gut microbiota impacts the host metabolome and affects its health span. How bacterial species in the gut influence age-dependent metabolic alteration has not been elucidated. Here we show in Drosophila melanogaster that allantoin, an end product of purine metabolism, is increased during aging in a microbiota-dependent manner. Allantoin levels are low in young flies but are commonly elevated upon lifespan-shortening dietary manipulations such as high-purine, high-sugar, or high-yeast feeding. Removing Acetobacter persici in the Drosophila microbiome attenuated age-dependent allantoin increase. Mono-association with A. persici, but not with Lactobacillus plantarum, increased allantoin in aged flies. A. persici increased allantoin via activation of innate immune signaling IMD pathway in the renal tubules. On the other hand, analysis of bacteria-conditioned diets revealed that L. plantarum can decrease allantoin by reducing purines in the diet. These data together demonstrate species-specific regulations of host purine levels by the gut microbiome. Gut bacterial species regulate the age-dependent metabolic shift in Drosophila Acetobacter persici increases purine levels via IMD activation in the renal tubules Lactobacillus plantarum decreases the purine levels in the Drosophila diet Diet- and bacteria-dependent elevation of purine levels may shorten the lifespan Biological Sciences; Molecular Biology; Immunology; Microbiome
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
通讯作者：
Benzer, Seymour
影响因子：
4.6
作者：
Guo Z;Zhang J;Wang Z;Ang KY;Huang S;Hou Q;Su X;Qiao J;Zheng Y;Wang L;Koh E;Danliang H;Xu J;Lee YK;Zhang H
通讯作者：
Zhang H
影响因子：
8.8
作者：
Clark RI;Salazar A;Yamada R;Fitz-Gibbon S;Morselli M;Alcaraz J;Rana A;Rera M;Pellegrini M;Ja WW;Walker DW
通讯作者：
Walker DW
DOI：
10.1093/gerona/57.10.m660
发表时间：
2002-10-01
影响因子：
5.1
作者：
Kuzuya, M;Ando, F;Shimokata, H
通讯作者：
Shimokata, H
影响因子：
5.3
作者：
Brankatschk, Marko;Eaton, Suzanne
通讯作者：
Eaton, Suzanne