Diminished levels of nasal S100A7 (psoriasin) in seasonal allergic rhinitis: an effect mediated by Th2 cytokines.
Diminished levels of nasal S100A7 (psoriasin) in seasonal allergic rhinitis: an effect mediated by Th2 cytokines.
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DOI：
10.1186/1465-9921-13-2
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发表时间：
2012-01-09
影响因子：
5.8
通讯作者：
Cardell LO
中科院分区：
文献类型：
--
作者：
Kvarnhammar AM;Rydberg C;Järnkrants M;Eriksson M;Uddman R;Benson M;Cardell LO
S100 A7是一种与多种炎症性疾病有关的抗菌肽。本研究旨在探讨S100 A7在季节性变应性鼻炎（SAR）中的表达及调控。鼻灌洗液（NAL）从健康对照组前和后脂多糖（LPS）激发，从SAR患者过敏原的挑战之前和之后，并从SAR患者完成过敏原特异性免疫治疗（ASIT）。从健康供体获得鼻活检、鼻上皮细胞和血液。气道上皮细胞系FaDu用于体外实验。采用实时定量RT-PCR和免疫组化方法检测S100 A7在鼻黏膜组织和细胞中的表达。通过ELISA测量NAL和培养物上清液中S100 A7的释放。在上皮细胞、中性粒细胞和外周血单个核细胞（PBMC）中研究重组S100 A7的功能。鼻内给药LPS诱导健康非过敏受试者S100 A7释放。SAR患者NAL中S100 A7的水平低于健康对照组，SAR组在过敏原激发后6 h进一步降低。相反，ASIT患者在完成治疗后显示出更高的水平。S100 A7在鼻粘膜上皮和腺体中均有表达，并由培养的上皮细胞分泌。用IL-4和组胺刺激抑制上皮S100 A7的释放。此外，重组S100 A7诱导中性粒细胞和PBMC的活化。本研究显示了在微生物刺激后鼻中S100 A7的上皮表达和分泌。在鼻炎患者和存在过敏性细胞因子的环境中，水平降低，表明SAR患者的抗菌防御受到损害。
S100A7 is an antimicrobial peptide involved in several inflammatory diseases. The aim of the present study was to explore the expression and regulation of S100A7 in seasonal allergic rhinitis (SAR). Nasal lavage (NAL) fluid was obtained from healthy controls before and after lipopolysaccharide (LPS) provocation, from SAR patients before and after allergen challenge, and from SAR patients having completed allergen-specific immunotherapy (ASIT). Nasal biopsies, nasal epithelial cells and blood were acquired from healthy donors. The airway epithelial cell line FaDu was used for in vitro experiments. Real-time RT-PCR and immunohistochemistry were used to determine S100A7 expression in nasal tissue and cells. Release of S100A7 in NAL and culture supernatants was measured by ELISA. The function of recombinant S100A7 was explored in epithelial cells, neutrophils and peripheral blood mononuclear cells (PBMC). Nasal administration of LPS induced S100A7 release in healthy non-allergic subjects. The level of S100A7 was lower in NAL from SAR patients than from healthy controls, and it was further reduced in the SAR group 6 h post allergen provocation. In contrast, ASIT patients displayed higher levels after completed treatment. S100A7 was expressed in the nasal epithelium and in glands, and it was secreted by cultured epithelial cells. Stimulation with IL-4 and histamine repressed the epithelial S100A7 release. Further, recombinant S100A7 induced activation of neutrophils and PBMC. The present study shows an epithelial expression and excretion of S100A7 in the nose after microbial stimulation. The levels are diminished in rhinitis patients and in the presence of an allergic cytokine milieu, suggesting that the antimicrobial defense is compromised in patients with SAR.
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影响因子：
10
作者：
Langley, SJ;Goldthorpe, S;Custovic, A
通讯作者：
Custovic, A
影响因子：
14.2
作者：
Ricca, V;Landi, M;Ciprandi, G
通讯作者：
Ciprandi, G
影响因子：
5.8
作者：
Bryborn M;Halldén C;Säll T;Adner M;Cardell LO
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Cardell LO
影响因子：
12.4
作者：
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通讯作者：
Cardell, L. O.
影响因子：
10.3
作者：
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通讯作者：
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