Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603.
Identification of the replication region in pBCNF5603, a bacteriocin-encoding plasmid, in the enterotoxigenic Clostridium perfringens strain F5603.
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DOI：
10.1186/s12866-015-0443-3
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发表时间：
2015-06-09
期刊：
影响因子：
4.2
通讯作者：
Nagahama M
中科院分区：
文献类型：
--
作者：
Miyamoto K;Seike S;Takagishi T;Okui K;Oda M;Takehara M;Nagahama M
最近对产气荚膜梭菌质粒的研究主要集中在毒素编码质粒或抗生素抗性质粒上。为了引起肠道疾病，产毒菌株必须在肠道内生长到足以产生足够毒素的水平，而这种体内生长通常涉及克服正常肠道微生物群。为此，细菌素的产生可能很重要。本研究将细菌素基因编码质粒pBCNF5603作为与肠毒素基因（enterotoxin gene, cpe）编码质粒共存质粒遗传分析的第一步，对其进行完全测序。该质粒与先前测序的两个产气荚膜衣原体质粒有一定的同源性，即携带cpb2基因的pCP13和携带bcn基因的pIP404。利用重组质粒，发现pCP13上与PCP63基因同源的rep基因具有功能。比较基因组学表明，在另外两个毒素质粒pCP-OS1和pCP-TS1上发现了rep基因的同源物。虽然重组质粒的功能分析表明，pBCNF5603和pCP13可能不相容，但单靠pBCNF5603的质粒复制和分区区不足以维持该质粒的稳定。这些结果表明，pBCNF5603是产气荚膜荚膜菌质粒与种间移动遗传元件之间的重组事件进化而来的。此外，编码bcn的质粒pBCNF5603可能被纳入Inc家族，该家族包括pCP13和两个变体iota编码质粒。此外，pBCNF5603上的bcn基因可能与产肠毒素产气荚膜梭菌引起的胃肠道疾病有关。本文的在线版本（doi:10.1186/s12866-015-0443-3）包含补充材料，可供授权用户使用。
Most recent studies of Clostridium perfringens plasmids have focused on toxin-encoding or antibiotic resistance plasmids. To cause intestinal disease, a toxigenic strain must grow in the intestines to levels allowing for sufficient toxin production and this in vivo growth often involves overcoming the normal intestinal microbial population. For this purpose, bacteriocin production might be important. In this study, as the first step in the genetic analysis of a co-existing plasmid with an enterotoxin gene (cpe)-encoding plasmid, the bacteriocin gene-encoding plasmid, pBCNF5603, was completely sequenced. This plasmid has some homology with two previously sequenced C. perfringens plasmids, namely, pCP13 carrying a cpb2 gene and pIP404 carrying a bcn gene. Using recombinant plasmids, the rep gene homologous to the PCP63 gene on pCP13 appeared to be functional. Comparative genomics indicated that the identified rep gene homologs were found on two additional toxin plasmids, pCP-OS1 and pCP-TS1. While functional analysis using recombinant plasmids indicated that pBCNF5603 and pCP13 are likely to be incompatible, the plasmid replication and partitioning region of pBCNF5603 alone was insufficient for stable maintenance of this plasmid. These findings suggest that pBCNF5603 evolved from recombination events between C. perfringens plasmids and inter-species mobile genetic element(s). In addition, the bcn-encoding plasmid, pBCNF5603, is likely to be included in the Inc family, which includes pCP13 and two variant iota-encoding plasmids. Furthermore, the bcn gene on pBCNF5603 could contribute to gastrointestinal disease induced by enterotoxigenic C. perfringens. The online version of this article (doi:10.1186/s12866-015-0443-3) contains supplementary material, which is available to authorized users.
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影响因子：
6.7
作者：
Keyburn AL;Boyce JD;Vaz P;Bannam TL;Ford ME;Parker D;Di Rubbo A;Rood JI;Moore RJ
通讯作者：
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影响因子：
56.9
作者：
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通讯作者：
Fraser, CM
DOI：
10.1073/pnas.182380799
发表时间：
2002-09-17
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
3.2
作者：
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通讯作者：
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影响因子：
3.7
作者：
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通讯作者：
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