The genomic basis of host and vector specificity in non-pathogenic trypanosomatids.
The genomic basis of host and vector specificity in non-pathogenic trypanosomatids.
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作者：
牛锥虫是一种非致病性的牛寄生虫，其表面蛋白结构可能有助于其在哺乳动物宿主体内的生存。它们的表面蛋白由占其基因组约10%的基因编码。绵羊的一种非致病性寄生虫，嗜黑锥虫，由羊羊传播，与T. theileri密切相关。为了探索这些物种之间宿主和载体的特异性，我们对噬噬T.基因组和转录组进行了测序，并组装了一个带注释的基因组草图。将嗜噬T.与43个动质体基因组进行了比较，其中包括T. theileri。嗜食锥虫和氏锥虫具有AT偏倚的基因组，这是已知锥虫中最大的偏倚。这种趋势可能是由于选择降低了基因组核苷酸的成本。嗜噬t菌的基因组比黑氏t菌的基因组小6.3Mb，并且在黑氏t菌中发现了预测表面特征的大家族蛋白缺失或大量减少。相反，噬t菌适度扩大了与避免补体介导的裂解相关的蛋白质家族。我们认为，这些物种的不同基因组特征与它们从昆虫媒介到哺乳动物宿主的传播方式有关。摘要：通过比较密切相关的非致病性锥虫，我们强调了细胞表面蛋白编码基因的差异投资，并预测这种差异投资与其宿主和载体的生活史有关。
Trypanosoma theileri, a non-pathogenic parasite of bovines, has a predicted surface protein architecture that likely aids survival in its mammalian host. Their surface proteins are encoded by genes which account for ∼10% of their genome. A non-pathogenic parasite of sheep, Trypanosoma melophagium, is transmitted by the sheep ked and is closely related to T. theileri. To explore host and vector specificity between these species, we sequenced the T. melophagium genome and transcriptome and an annotated draft genome was assembled. T. melophagium was compared to 43 kinetoplastid genomes, including T. theileri. T. melophagium and T. theileri have an AT biased genome, the greatest bias of publicly available trypanosomatids. This trend may result from selection acting to decrease the genomic nucleotide cost. The T. melophagium genome is 6.3Mb smaller than T. theileri and large families of proteins, characteristic of the predicted surface of T. theileri, were found to be absent or greatly reduced in T. melophagium. Instead, T. melophagium has modestly expanded protein families associated with the avoidance of complement-mediated lysis. We propose that the contrasting genomic features of these species is linked to their mode of transmission from their insect vector to their mammalian host. Summary: Comparing closely related non-pathogenic trypanosomes, we highlight differential investment in cell-surface protein encoding genes and predict this differential investment is associated with the life histories of their hosts and vectors.
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影响因子：
5.8
作者：
Barnett, Derek W.;Garrison, Erik K.;Marth, Gabor T.
通讯作者：
Marth, Gabor T.
DOI：
10.1083/jcb.201506114
发表时间：
2015-10-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Domingo-Sananes MR;Szöőr B;Ferguson MA;Urbaniak MD;Matthews KR
通讯作者：
Matthews KR
影响因子：
3.3
作者：
Cavalier-Smith, Thomas
通讯作者：
Cavalier-Smith, Thomas
影响因子：
2.7
作者：
Cabanettes F;Klopp C
通讯作者：
Klopp C
影响因子：
2.2
作者：
DOHERTY, ML;WINDLE, H;MURRAY, M
通讯作者：
MURRAY, M