Characteristics of N(6)-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii.
Characteristics of N(6)-methyladenosine Modification During Sexual Reproduction of Chlamydomonas reinhardtii.
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莱茵衣藻有性繁殖过程中N6-甲基腺苷修饰特征
DOI：
10.1016/j.gpb.2022.04.004
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发表时间：
2023-08
影响因子：
9.5
通讯作者：
Yang, Wenqiang
中科院分区：
文献类型：
--
作者：
Lv, Ying;Han, Fei;Liu, Mengxia;Zhang, Ting;Cui, Guanshen;Wang, Jiaojiao;Yang, Ying;Yang, Yun-Gui;Yang, Wenqiang
关键词：
单细胞绿色衣藻莱茵衣藻（Chlamyeliumreinhardtii，以下简称衣藻）兼具植物和动物的特性，是研究光合作用、有性生殖和生命周期等基本过程的理想模式生物。N6-甲基腺苷（m6 A）是最常见的mRNA修饰，在动植物有性生殖过程中起着重要作用。然而，在衣原体有性生殖过程中m6 A修饰的模式和功能仍然未知。在这里，我们进行了转录组和甲基化RNA免疫沉淀测序（MeRIP-seq）分析6个样本从不同阶段的衣原体生命周期的有性生殖。结果表明，在衣原体mRNA中，m6 A的修饰主要发生在C端（D = G/A/U，R = A/G）。此外，衣原体m6 A主要富集在3′非翻译区（3′ UTR），并与各阶段转录本的丰度呈负相关。特别是，有一个显着的负相关性之间的表达水平和m6 A水平的基因参与的微管相关途径，表明m6 A修饰影响有性生殖和生命周期的衣原体通过调节微管为基础的运动。总之，我们的研究结果是第一个展示的分布和功能的衣原体m6 A修饰的mRNA，并提供新的进化见解m6 A修饰在其他植物生物的有性生殖过程中。
The unicellular green alga Chlamydomonas reinhardtii (hereafter Chlamydomonas) possesses both plant and animal attributes, and it is an ideal model organism for studying fundamental processes such as photosynthesis, sexual reproduction, and life cycle. N6-methyladenosine (m6A) is the most prevalent mRNA modification, and it plays important roles during sexual reproduction in animals and plants. However, the pattern and function of m6A modification during the sexual reproduction of Chlamydomonas remain unknown. Here, we performed transcriptome and methylated RNA immunoprecipitation sequencing (MeRIP-seq) analyses on six samples from different stages during sexual reproduction of the Chlamydomonas life cycle. The results show that m6A modification frequently occurs at the main motif of DRAC (D = G/A/U, R = A/G) in Chlamydomonas mRNAs. Moreover, m6A peaks in Chlamydomonas mRNAs are mainly enriched in the 3′ untranslated regions (3′UTRs) and negatively correlated with the abundance of transcripts at each stage. In particular, there is a significant negative correlation between the expression levels and the m6A levels of genes involved in the microtubule-associated pathway, indicating that m6A modification influences the sexual reproduction and the life cycle of Chlamydomonas by regulating microtubule-based movement. In summary, our findings are the first to demonstrate the distribution and the functions of m6A modification in Chlamydomonas mRNAs and provide new evolutionary insights into m6A modification in the process of sexual reproduction in other plant organisms.
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YTHDF2 通过直接招募 CCR4-NOT 去腺苷酶复合物来破坏含有 m(6)A 的 RNA 的稳定性。
影响因子：
16.6
作者：
Du, Hao;Zhao, Ya;He, Jinqiu;Zhang, Yao;Xi, Hairui;Liu, Mofang;Ma, Jinbiao;Wu, Ligang
通讯作者：
Wu, Ligang
影响因子：
16
作者：
Heinz S;Benner C;Spann N;Bertolino E;Lin YC;Laslo P;Cheng JX;Murre C;Singh H;Glass CK
通讯作者：
Glass CK
DOI：
10.1111/tpj.12795
发表时间：
2015-05
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Cross FR;Umen JG
通讯作者：
Umen JG
影响因子：
56.9
作者：
Frayling, Timothy M.;Timpson, Nicholas J.;McCarthy, Mark I.
通讯作者：
McCarthy, Mark I.
DOI：
10.1083/jcb.141.4.993
发表时间：
1998-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cole DG;Diener DR;Himelblau AL;Beech PL;Fuster JC;Rosenbaum JL
通讯作者：
Rosenbaum JL