High Level of Neutrophil Extracellular Traps Correlates With Poor Prognosis of Severe Influenza A Infection
High Level of Neutrophil Extracellular Traps Correlates With Poor Prognosis of Severe Influenza A Infection
复制标题
高水平的中性粒细胞胞外陷阱与严重甲型流感感染的不良预后相关
DOI：
10.1093/infdis/jix475
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发表时间：
2018-01
影响因子：
6.4
通讯作者：
Zeng Hui
中科院分区：
文献类型：
--
作者：
Zhu Liuluan;Liu Lu;Zhang Yue;Pu Lin;Liu Jingyuan;Li Xingwang;Chen Zhihai;Hao Yu;Wang Beibei;Han Junyan;Li Guoli;Liang Shuntao;Xiong Haofeng;Zheng Hong;Li Ang;Xu Jianqing;Zeng Hui
背景：大多数甲型流感病毒（IAV）严重感染患者会进展为急性呼吸窘迫综合征，甚至多器官功能障碍综合征（MODS）。中性粒细胞胞外诱捕网（NETs）可由病原体诱导，并且……（原句此处“resp”不完整）
Background
Most patients with severe infection with influenza A virus (IAV) progress to acute respiratory distress syndrome and even multiple organ dysfunction syndrome (MODS). Neutrophil extracellular traps (NETs) can be induced by pathogens and are responsible for immune tissue damage. We conducted a prospective study on the production and effects of NETs in H7N9 and H1N1 patients.
Methods
We investigated NET production in plasma and supernatant of cultured neutrophils by measuring cell-free deoxyribonucleic acid (DNA) and myeloperoxidase (MPO)-DNA complexes with PicoGreen dye and enzyme-linked immunosorbent assay methods, respectively. We also observed NET structure by immunofluorescence staining.
Results
We found that patients with severe influenza showed elevated plasma NET level on the day of admission. Neutrophils from these patients showed higher capacity to release MPO-DNA complex in response to interleukin-8 or lipopolysaccharide stimulation. We also found that NETs from H7N9 and H1N1 patients increased the permeability of alveolar epithelial cells, and, consequently, NET production was positively correlated with acute physiology and chronic health evaluation (APACHE) II score and MODS.
Conclusions
These data indicate that high level of NETs contributes to lung injury and is correlated with severity of disease. Thus, NETs might be a key factor to predict the poor prognosis in IAV patients.
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影响因子：
5.2
作者：
von Köckritz-Blickwede M;Blodkamp S;Nizet V
通讯作者：
Nizet V
DOI：
10.1126/stke.3792007pe11
发表时间：
2007-03
期刊：
Science's STKE
影响因子：
--
作者：
B. Steinberg;S. Grinstein
通讯作者：
B. Steinberg;S. Grinstein
影响因子：
4.8
作者：
De Filippo, Katia;Neill, Daniel R.;Hogg, Nancy
通讯作者：
Hogg, Nancy
DOI：
10.1016/s0084-3954(09)79641-1
发表时间：
2011
期刊：
Yearbook of Pediatrics
影响因子：
--
作者：
J. Stockman
通讯作者：
J. Stockman
影响因子：
6
作者：
Parkos, Charles A.
通讯作者：
Parkos, Charles A.