Establishing the proteome of normal human cerebrospinal fluid.
Establishing the proteome of normal human cerebrospinal fluid.
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DOI：
10.1371/journal.pone.0010980
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发表时间：
2010-06-11
期刊：
影响因子：
3.7
通讯作者：
Bergquist J
中科院分区：
文献类型：
--
作者：
Schutzer SE;Liu T;Natelson BH;Angel TE;Schepmoes AA;Purvine SO;Hixson KK;Lipton MS;Camp DG;Coyle PK;Smith RD;Bergquist J
了解正常人脑脊液（CSF）的整个蛋白质含量（蛋白质组）将有助于深入了解神经和精神疾病。到目前为止，技术障碍和获得真正的正常样本阻碍了这一目标的实现。我们应用免疫亲和分离和高灵敏度和高分辨率液相色谱-质谱法检测健康正常人的脑脊液。在正常受试者的CSF中鉴定了2630种蛋白质，其中56%是CSF特异性的，在我们在血浆中鉴定的3654种蛋白质的更大集合中没有发现。我们还检查了来自先前由其他人检查的受试者组的CSF，作为正常人的替代物，其中神经系统症状需要腰椎穿刺，但临床实验室报告为正常。我们发现他们的CSF蛋白质和我们的非神经系统正常人之间存在统计学显著差异。我们还检查了10名志愿受试者的CSF，这些受试者至少间隔4周进行腰椎穿刺，发现与受试者相比，个体的CSF蛋白质几乎没有变化。我们的研究结果代表了迄今为止真正正常CSF的最全面表征。这种正常的CSF蛋白质组为研究具有神经和精神特征的各种疾病建立了比较标准和基础。
Knowledge of the entire protein content, the proteome, of normal human cerebrospinal fluid (CSF) would enable insights into neurologic and psychiatric disorders. Until now technologic hurdles and access to true normal samples hindered attaining this goal. We applied immunoaffinity separation and high sensitivity and resolution liquid chromatography-mass spectrometry to examine CSF from healthy normal individuals. 2630 proteins in CSF from normal subjects were identified, of which 56% were CSF-specific, not found in the much larger set of 3654 proteins we have identified in plasma. We also examined CSF from groups of subjects previously examined by others as surrogates for normals where neurologic symptoms warranted a lumbar puncture but where clinical laboratory were reported as normal. We found statistically significant differences between their CSF proteins and our non-neurological normals. We also examined CSF from 10 volunteer subjects who had lumbar punctures at least 4 weeks apart and found that there was little variability in CSF proteins in an individual as compared to subject to subject. Our results represent the most comprehensive characterization of true normal CSF to date. This normal CSF proteome establishes a comparative standard and basis for investigations into a variety of diseases with neurological and psychiatric features.
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