dnaMATE: a consensus melting temperature prediction server for short DNA sequences.
dnaMATE: a consensus melting temperature prediction server for short DNA sequences.
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DOI：
10.1093/nar/gki379
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发表时间：
2005-07-01
影响因子：
14.9
通讯作者：
Melo F
中科院分区：
文献类型：
--
作者：
Panjkovich A;Norambuena T;Melo F
针对短DNA序列的精确且鲁棒的大规模解链温度预测服务器被分派。服务器使用基于三个独立的热力学数据表的最近邻模型计算共有熔化温度值。一致性方法给出了解链温度的准确预测，正如最近在使用16-30 nt长度范围内的DNA序列的所有可用实验数据进行的基准测试中所证明的那样。这构成了第一个网络服务器，已经实现了在真实的时间（高达5000个DNA序列可以提交在一个单一的运行）进行大规模的解链温度计算。该服务器在50-600 mM单价盐浓度范围内进行的计算的预期准确度是89%的熔融温度预测将与实验数据具有<5°C的误差或偏差。服务器可以自由访问。该软件的独立可执行版本的Linux，Macintosh和Windows平台上也可以免费获得在同一网站。支持这一服务器的进一步详细信息可在上述同一网站上查阅。
An accurate and robust large-scale melting temperature prediction server for short DNA sequences is dispatched. The server calculates a consensus melting temperature value using the nearest-neighbor model based on three independent thermodynamic data tables. The consensus method gives an accurate prediction of melting temperature, as it has been recently demonstrated in a benchmark performed using all available experimental data for DNA sequences within the length range of 16–30 nt. This constitutes the first web server that has been implemented to perform a large-scale calculation of melting temperatures in real time (up to 5000 DNA sequences can be submitted in a single run). The expected accuracy of calculations carried out by this server in the range of 50–600 mM monovalent salt concentration is that 89% of the melting temperature predictions will have an error or deviation of <5°C from experimental data. The server can be freely accessed at . The standalone executable versions of this software for LINUX, Macintosh and Windows platforms are also freely available at the same web site. Detailed further information supporting this server is available at the same web site referenced above.
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影响因子：
14.9
作者：
Chiu, WLAK;Sze, CN;Au-Yeung, SCF
通讯作者：
Au-Yeung, SCF
DOI：
10.1073/pnas.83.11.3746
发表时间：
1986-06-01
影响因子：
11.1
作者：
BRESLAUER, KJ;FRANK, R;MARKY, LA
通讯作者：
MARKY, LA
DOI：
10.1073/pnas.95.4.1460
发表时间：
1998-02-17
影响因子：
11.1
作者：
SantaLucia, J
通讯作者：
SantaLucia, J
影响因子：
2.9
作者：
SantaLucia, J;Allawi, HT;Seneviratne, A
通讯作者：
Seneviratne, A
影响因子：
14.9
作者：
Sugimoto, N;Nakano, S;Honda, K
通讯作者：
Honda, K